Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Mus musculus | 5743 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | Mus musculus | - |
NADP+ + NADH | - |
r | |
NADPH + NAD+ | Mus musculus C57/BL6J | - |
NADP+ + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus C57/BL6J | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ | - |
Mus musculus | NADP+ + NADH | - |
r | |
NADPH + NAD+ | - |
Mus musculus C57/BL6J | NADP+ + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NNT | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | spontaneous C57BL/6J(B6J-NntMUT) mutant mice show major redox alterations in respiring mitchondria, including an absence of transhydrogenation between NAD+ and NADP+, higher rates of H2O2 release, the spontaneous oxidation of NADPH,the poor ability to metabolize organic peroxide, and a higher susceptibility to undergo Ca2+-induced mitochondrial permeability transition. Liver mitochondria from B6J-NntMUT mice do not possess NNT activity. The mitochondria of mutant B6J-NntMUT mice exhibit increased oxidized/reduced glutathione ratios as compared to wild-type B6JUnib-NntW mice, phenotypes, overview | Mus musculus |
physiological function | the enzyme functions as a high-capacity source of mitochondrial NADPH and that its functional loss due to the Nnt mutation results in mitochondrial redox abnormalities, most notably a poor ability to sustain NADP+ and glutathione in the reduced states. The enzyme is assembled across the inner mitochondrial membrane and translocates H+ to the mitochondrial matrix as NADP+ is reduced at the expense of NADH on the matrix side. The electrochemical gradient across the inner mitochondrial membrane shifts the NNT equilibrium to the right | Mus musculus |