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Literature summary for 1.6.1.1 extracted from

  • Wermuth, B.; Kaplan, N.O.
    Pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa: purification by affinity chromatography and physicochemical properties (1976), Arch. Biochem. Biophys., 176, 136-143.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
2'-AMP
-
 Pseudomonas aeruginosa

General Stability

General Stability Organism
urea, 8 M, no dissociation, complete inactivation of enzyme activity in 5 M urea Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Pseudomonas aeruginosa
1600000
-
 sedimentation equilibrium in presence of 2'-AMP Pseudomonas aeruginosa
6400000
-
 sedimentation equilibrium in presence of NADP+ Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on immobilized 2'-AMP Pseudomonas aeruginosa

Renatured (Commentary)

Renatured (Comment) Organism
urea inactivation: 3% reactivation after dialysis against buffer containing 0.1 mM FAD, 35% after dialysis against buffer containing 1% mercaptoethanol and 95% reactivation after dialysis against buffer containing both 0.1 mM FAD and 1% mercaptoethanol Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
-
 Pseudomonas aeruginosa

Subunits

Subunits Comment Organism
octamer x * 54000, SDS-PAGE Pseudomonas aeruginosa