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Literature summary for 1.5.99.B2 extracted from

  • Kawakami, R.; Sakuraba, H.; Tsuge, H.; Goda, S.; Katunuma, N.; Ohshima, T.
    A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 (2005), FEBS J., 272, 4044-4054.
    View publication on PubMed
Search for more literature for 1.5.99.B2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 2,6-dichlorophenolindophenol isoform PDH1 Pyrococcus horikoshii
4
-
 L-proline isoform PDH1 Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
20000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
43000
-
 isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii
46000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
52000
-
 isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
56000
-
 isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii
101000
-
 isoform PDH2, gel filtration Pyrococcus horikoshii
440000
-
 isoform PDH1, gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
 isoforms PDH1 and PDH2
-
Pyrococcus horikoshii OT-3
-
 isoforms PDH1 and PDH2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.939
-
 isoform PDH1 Pyrococcus horikoshii
1.4
-
 isoform PDH2 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Pyrococcus horikoshii (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Pyrococcus horikoshii OT-3 (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid Pyrococcus horikoshii ?
-
 ?
additional information isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid Pyrococcus horikoshii OT-3 ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer isoform PDH2, 1 * 52000, alpha subunit, + 1 * 46000, beta subunit + 1 * 20000, gamma subunit, + 1 * 8000, delta subunit, SDS-PAGE Pyrococcus horikoshii
octamer isoform PDH1, 4 * 56000, alpha subunit + 4 * 43000, beta subunit, SDS-PAGE Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 isoform PDH1, stable for at least 120 min Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 isoform PDH1 Pyrococcus horikoshii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 isoform PDH1, 50°C, stable for at least 30 min Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ATP isoform PDH1, 1 mol per mol of alpha subunit Pyrococcus horikoshii
FAD isoform PDH1, 1 mol per mol of beta subunit, alpha and beta subunits separatively produced in Escherichia coli cannot bind FAD Pyrococcus horikoshii
FMN situated at the interface of alpha and beta subunit Pyrococcus horikoshii