BRENDA - Enzyme Database
show all sequences of 1.5.99.6

Purification and characterization of soybean cotyledonary spermidine dehydrogenase

Park, S.J.; Cho, Y.D.; J. Biochem. Mol. Biol. 28, 408-413 (1995)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
diaminopropane
-
Glycine max
spermidine
-
Glycine max
Inhibitors
Inhibitors
Commentary
Organism
Structure
DTNB
-
Glycine max
N-ethylmaleimide
-
Glycine max
p-chloromercuribenzoate
-
Serratia marcescens
p-chloromercuribenzoate
-
Glycine max
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
spermidine
-
Pseudomonas aeruginosa
0.61
-
spermidine
-
Glycine max
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
2 * 65000, SDS-PAGE
Glycine max
130000
-
gel filtration
Glycine max
Organism
Organism
UniProt
Commentary
Textmining
Glycine max
-
soybean
-
Pseudomonas aeruginosa
-
-
-
Serratia marcescens
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Serratia marcescens
-
Pseudomonas aeruginosa
-
Glycine max
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
800
-
-
Glycine max
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
spermidine + electron acceptor
-
392554
Glycine max
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392554
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392554
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 65000, SDS-PAGE
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Glycine max
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
diaminopropane
-
Glycine max
spermidine
-
Glycine max
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DTNB
-
Glycine max
N-ethylmaleimide
-
Glycine max
p-chloromercuribenzoate
-
Serratia marcescens
p-chloromercuribenzoate
-
Glycine max
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.037
-
spermidine
-
Pseudomonas aeruginosa
0.61
-
spermidine
-
Glycine max
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
2 * 65000, SDS-PAGE
Glycine max
130000
-
gel filtration
Glycine max
Purification (Commentary) (protein specific)
Commentary
Organism
-
Serratia marcescens
-
Pseudomonas aeruginosa
-
Glycine max
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
800
-
-
Glycine max
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
spermidine + electron acceptor
-
392554
Glycine max
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392554
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392554
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 65000, SDS-PAGE
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.3
-
-
Glycine max
Other publictions for EC 1.5.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
675842
Dasu
Characterization and a role of ...
Pseudomonas aeruginosa
Microbiology
152
2265-2272
2006
-
-
1
-
-
-
-
1
1
-
1
1
-
3
-
-
1
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392554
Park
-
Purification and characterizat ...
Glycine max, Pseudomonas aeruginosa, Serratia marcescens
J. Biochem. Mol. Biol.
28
408-413
1995
2
-
-
-
-
-
5
2
-
-
2
-
-
3
-
-
3
-
-
1
1
-
3
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
-
-
2
-
-
-
-
3
-
1
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392553
Hisano
Characterization of membrane-b ...
Citrobacter freundii, Citrobacter freundii IFO12681
Biosci. Biotechnol. Biochem.
56
1916-1920
1992
-
-
-
1
-
-
-
-
2
-
1
-
-
5
-
-
1
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392551
Hisano
-
Effect of bivalent metal ions ...
Citrobacter freundii, Citrobacter freundii IFO12681, Pseudomonas aeruginosa, Serratia marcescens
J. Ferment. Bioeng.
70
280-282
1990
-
-
-
-
-
-
-
-
-
3
-
-
-
6
-
-
3
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392552
Hisano
-
Microbial spermidine dehydroge ...
Citrobacter freundii, Citrobacter freundii IFO12681, Flavobacterium dormitator, Pseudomonas aeruginosa, Pseudomonas aeruginosa IFO3080, Serratia marcescens, Serratia marcescens IFO3046
J. Ferment. Bioeng.
69
335-340
1990
-
-
-
-
-
-
9
5
-
-
5
-
-
9
-
-
3
-
-
-
2
-
25
3
-
2
-
2
-
3
-
2
2
-
-
-
-
-
-
2
-
-
-
-
9
-
5
-
-
5
-
-
-
-
3
-
-
2
-
25
3
2
-
2
-
3
-
2
-
-
-
-
-
-
-
392550
Okada
Purification of spermidine deh ...
Serratia marcescens
Methods Enzymol.
94
303-305
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392548
Okada
Affinity chromatography of put ...
Serratia marcescens
J. Biochem.
85
1225-1233
1979
-
-
-
-
-
-
2
1
-
-
1
-
-
3
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392549
Okada
Substrate binding characterist ...
Serratia marcescens
J. Biochem.
85
1235-1243
1979
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
392545
Tabor
Biosynthesis and metabolism of ...
Serratia marcescens
Adv. Enzymol. Relat. Areas Mol. Biol.
36
203-268
1972
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392547
Tabor
Spermidine dehydrogenase (Serr ...
Serratia marcescens
Methods Enzymol.
17B
746-753
1971
-
-
-
-
-
-
3
2
-
-
1
-
-
2
-
-
-
-
-
-
1
1
8
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
1
-
-
-
-
-
-
-
1
1
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
392546
Tabor
Identification of flavin adeni ...
Serratia marcescens
J. Biol. Chem.
245
5424-5433
1970
-
-
-
-
-
-
-
-
-
-
2
1
-
2
-
-
1
-
-
-
1
1
10
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
1
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-