BRENDA - Enzyme Database
show all sequences of 1.5.99.6

Microbial spermidine dehydrogenase: Purification and properties of the enzyme in Pseudomonas aeruginosa and Citrobacter freundii

Hisano, T.; Abe, S.; Wakashiro, M.; Kimura, A.; Murata, K.; J. Ferment. Bioeng. 69, 335-340 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
-
Citrobacter freundii
Hg2+
-
Pseudomonas aeruginosa
HgCl2
inhibition almost complete
Citrobacter freundii
HgCl2
inhibition by 30%
Pseudomonas aeruginosa
p-chloromercuribenzoate
slight inhibition
Citrobacter freundii
p-chloromercuribenzoate
slight inhibition
Pseudomonas aeruginosa
p-chloromercuribenzoate
-
Serratia marcescens
Quinacrine
-
Citrobacter freundii
Quinacrine
-
Serratia marcescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0104
-
spermine
enzyme I
Citrobacter freundii
0.0143
-
spermine
enzyme I
Citrobacter freundii
0.0183
-
spermidine
enzyme II
Citrobacter freundii
0.0244
-
spermidine
enzyme I
Citrobacter freundii
0.037
-
spermidine
-
Pseudomonas aeruginosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Pseudomonas aeruginosa
43000
-
gel filtration
Citrobacter freundii
63000
-
SDS-PAGE
Pseudomonas aeruginosa
63000
-
SDS-PAGE
Citrobacter freundii
76000
-
-
Serratia marcescens
Organism
Organism
UniProt
Commentary
Textmining
Citrobacter freundii
-
IFO12681
-
Citrobacter freundii IFO12681
-
IFO12681
-
Flavobacterium dormitator
-
var.
-
Pseudomonas aeruginosa
-
IFO3080
-
Pseudomonas aeruginosa IFO3080
-
IFO3080
-
Serratia marcescens
-
IFO3046
-
Serratia marcescens IFO3046
-
IFO3046
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas aeruginosa
-
Serratia marcescens
-
Citrobacter freundii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.52
-
-
Pseudomonas aeruginosa
97.5
-
-
Citrobacter freundii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
spermidine + 2,6-dichlorophenolindophenol
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + electron acceptor
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Flavobacterium dormitator
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermine + electron acceptor
-
392552
Pseudomonas aeruginosa
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Citrobacter freundii
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Citrobacter freundii IFO12681
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Pseudomonas aeruginosa IFO3080
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Serratia marcescens IFO3046
? + reduced electron acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
-
Serratia marcescens
monomer
1 * 63000, SDS-PAGE
Pseudomonas aeruginosa
monomer
1 * 63000, SDS-PAGE
Citrobacter freundii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
30
-
Pseudomonas aeruginosa
50
-
-
Citrobacter freundii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
stable below
Pseudomonas aeruginosa
50
-
stable below
Citrobacter freundii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Citrobacter freundii
7.2
-
-
Serratia marcescens
8
-
-
Pseudomonas aeruginosa
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
-
Pseudomonas aeruginosa
7
8
-
Citrobacter freundii
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Serratia marcescens
heme
-
Citrobacter freundii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Serratia marcescens
heme
-
Citrobacter freundii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
-
Pseudomonas aeruginosa
Hg2+
-
Citrobacter freundii
HgCl2
inhibition by 30%
Pseudomonas aeruginosa
HgCl2
inhibition almost complete
Citrobacter freundii
p-chloromercuribenzoate
-
Serratia marcescens
p-chloromercuribenzoate
slight inhibition
Pseudomonas aeruginosa
p-chloromercuribenzoate
slight inhibition
Citrobacter freundii
Quinacrine
-
Serratia marcescens
Quinacrine
-
Citrobacter freundii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0104
-
spermine
enzyme I
Citrobacter freundii
0.0143
-
spermine
enzyme I
Citrobacter freundii
0.0183
-
spermidine
enzyme II
Citrobacter freundii
0.0244
-
spermidine
enzyme I
Citrobacter freundii
0.037
-
spermidine
-
Pseudomonas aeruginosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration
Pseudomonas aeruginosa
43000
-
gel filtration
Citrobacter freundii
63000
-
SDS-PAGE
Pseudomonas aeruginosa
63000
-
SDS-PAGE
Citrobacter freundii
76000
-
-
Serratia marcescens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas aeruginosa
-
Serratia marcescens
-
Citrobacter freundii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.52
-
-
Pseudomonas aeruginosa
97.5
-
-
Citrobacter freundii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
spermidine + 2,6-dichlorophenolindophenol
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + electron acceptor
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Flavobacterium dormitator
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + electron acceptor
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Pseudomonas aeruginosa
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Citrobacter freundii
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Citrobacter freundii IFO12681
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Pseudomonas aeruginosa IFO3080
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + K3Fe(CN)6
-
392552
Serratia marcescens IFO3046
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermine + electron acceptor
-
392552
Pseudomonas aeruginosa
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Citrobacter freundii
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Citrobacter freundii IFO12681
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Pseudomonas aeruginosa IFO3080
? + reduced electron acceptor
-
-
-
?
spermine + electron acceptor
-
392552
Serratia marcescens IFO3046
? + reduced electron acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Serratia marcescens
monomer
1 * 63000, SDS-PAGE
Pseudomonas aeruginosa
monomer
1 * 63000, SDS-PAGE
Citrobacter freundii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
30
-
Pseudomonas aeruginosa
50
-
-
Citrobacter freundii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
stable below
Pseudomonas aeruginosa
50
-
stable below
Citrobacter freundii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Citrobacter freundii
7.2
-
-
Serratia marcescens
8
-
-
Pseudomonas aeruginosa
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
10
-
Pseudomonas aeruginosa
7
8
-
Citrobacter freundii
Other publictions for EC 1.5.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
675842
Dasu
Characterization and a role of ...
Pseudomonas aeruginosa
Microbiology
152
2265-2272
2006
-
-
1
-
-
-
-
1
1
-
1
1
-
7
-
-
1
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392554
Park
-
Purification and characterizat ...
Glycine max, Pseudomonas aeruginosa, Serratia marcescens
J. Biochem. Mol. Biol.
28
408-413
1995
2
-
-
-
-
-
5
2
-
-
2
-
-
3
-
-
3
-
-
1
1
-
3
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
-
-
2
-
-
-
-
3
-
1
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392553
Hisano
Characterization of membrane-b ...
Citrobacter freundii, Citrobacter freundii IFO12681
Biosci. Biotechnol. Biochem.
56
1916-1920
1992
-
-
-
1
-
-
-
-
2
-
1
-
-
9
-
-
1
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392551
Hisano
-
Effect of bivalent metal ions ...
Citrobacter freundii, Pseudomonas aeruginosa, Serratia marcescens, Citrobacter freundii IFO12681
J. Ferment. Bioeng.
70
280-282
1990
-
-
-
-
-
-
-
-
-
3
-
-
-
6
-
-
3
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392552
Hisano
-
Microbial spermidine dehydroge ...
Citrobacter freundii, Flavobacterium dormitator, Pseudomonas aeruginosa, Serratia marcescens, Citrobacter freundii IFO12681, Pseudomonas aeruginosa IFO3080, Serratia marcescens IFO3046
J. Ferment. Bioeng.
69
335-340
1990
-
-
-
-
-
-
9
5
-
-
5
-
-
9
-
-
3
-
-
-
2
-
25
3
-
2
-
2
-
3
-
2
2
-
-
-
-
-
-
2
-
-
-
-
9
-
5
-
-
5
-
-
-
-
3
-
-
2
-
25
3
2
-
2
-
3
-
2
-
-
-
-
-
-
-
392550
Okada
Purification of spermidine deh ...
Serratia marcescens
Methods Enzymol.
94
303-305
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392548
Okada
Affinity chromatography of put ...
Serratia marcescens
J. Biochem.
85
1225-1233
1979
-
-
-
-
-
-
2
1
-
-
1
-
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7
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-
1
-
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1
-
3
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
1
-
-
1
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3
1
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392549
Okada
Substrate binding characterist ...
Serratia marcescens
J. Biochem.
85
1235-1243
1979
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4
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4
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-
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-
1
-
2
-
-
-
-
-
-
2
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-
-
4
-
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-
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-
-
-
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4
4
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-
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-
-
-
-
-
-
-
1
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2
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-
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-
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2
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392545
Tabor
Biosynthesis and metabolism of ...
Serratia marcescens
Adv. Enzymol. Relat. Areas Mol. Biol.
36
203-268
1972
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-
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1
1
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1
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1
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-
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-
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8
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-
-
-
-
-
-
3
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-
-
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-
-
3
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-
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-
-
-
-
-
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1
1
-
-
-
1
-
-
-
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8
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392547
Tabor
Spermidine dehydrogenase (Serr ...
Serratia marcescens
Methods Enzymol.
17B
746-753
1971
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-
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3
2
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1
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4
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1
1
8
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-
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-
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2
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2
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2
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3
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2
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1
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1
1
8
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2
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392546
Tabor
Identification of flavin adeni ...
Serratia marcescens
J. Biol. Chem.
245
5424-5433
1970
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2
1
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3
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1
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1
1
10
1
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1
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3
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-
-
3
-
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-
-
-
-
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2
1
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1
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1
1
10
1
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1
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