BRENDA - Enzyme Database
show all sequences of 1.5.99.6

Identification of flavin adenine dinucleotide and heme in a homogeneous spermidine dehydrogenase from Serratia marcescens

Tabor, C.W.; Kellog, P.D.; J. Biol. Chem. 245, 5424-5433 (1970)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
sedimentation equilibrium centrifugation, centrifugation in a sucrose density gradient
Serratia marcescens
79000
-
sedimentation velocity centrifugation, diffusion coefficient
Serratia marcescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
spermidine + cytochrome c
Serratia marcescens
natural electron acceptor horse heart cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Serratia marcescens
-
ATCC 25179
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Serratia marcescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
420
-
-
Serratia marcescens
Storage Stability
Storage Stability
Organism
-20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of actvity
Serratia marcescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
monoacetylspermidine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N-(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + cytochrome c
natural electron acceptor horse heart cytochrome c
392546
Serratia marcescens
1,3-diaminopropane + gamma-aminobutylaldehyde
-
-
-
?
spermidine + electron acceptor
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + K3Fe(CN)6
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + phenazine methosulfate
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
-
-
-
?
spermine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 76000, SDS-PAGE
Serratia marcescens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
58
-
stable when heated for 5 min
Serratia marcescens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Serratia marcescens
heme
-
Serratia marcescens
iron-protoporphyrin IX
-
Serratia marcescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Serratia marcescens
heme
-
Serratia marcescens
iron-protoporphyrin IX
-
Serratia marcescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76000
-
sedimentation equilibrium centrifugation, centrifugation in a sucrose density gradient
Serratia marcescens
79000
-
sedimentation velocity centrifugation, diffusion coefficient
Serratia marcescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
spermidine + cytochrome c
Serratia marcescens
natural electron acceptor horse heart cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Serratia marcescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
420
-
-
Serratia marcescens
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of actvity
Serratia marcescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
monoacetylspermidine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N-(3-aminopropyl)-1,3-propanediamine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
?
spermidine + cytochrome c
natural electron acceptor horse heart cytochrome c
392546
Serratia marcescens
1,3-diaminopropane + gamma-aminobutylaldehyde
-
-
-
?
spermidine + electron acceptor
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
?
spermidine + K3Fe(CN)6
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
?
spermidine + phenazine methosulfate
-
392546
Serratia marcescens
1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
-
-
-
?
spermine + electron acceptor
-
392546
Serratia marcescens
? + reduced electron acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 76000, SDS-PAGE
Serratia marcescens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
58
-
stable when heated for 5 min
Serratia marcescens
Other publictions for EC 1.5.99.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
675842
Dasu
Characterization and a role of ...
Pseudomonas aeruginosa
Microbiology
152
2265-2272
2006
-
-
1
-
-
-
-
1
1
-
1
1
-
7
-
-
1
-
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392554
Park
-
Purification and characterizat ...
Glycine max, Pseudomonas aeruginosa, Serratia marcescens
J. Biochem. Mol. Biol.
28
408-413
1995
2
-
-
-
-
-
5
2
-
-
2
-
-
3
-
-
3
-
-
1
1
-
3
1
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
-
-
2
-
-
-
-
3
-
1
1
-
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
392553
Hisano
Characterization of membrane-b ...
Citrobacter freundii, Citrobacter freundii IFO12681
Biosci. Biotechnol. Biochem.
56
1916-1920
1992
-
-
-
1
-
-
-
-
2
-
1
-
-
9
-
-
1
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392551
Hisano
-
Effect of bivalent metal ions ...
Citrobacter freundii, Pseudomonas aeruginosa, Serratia marcescens, Citrobacter freundii IFO12681
J. Ferment. Bioeng.
70
280-282
1990
-
-
-
-
-
-
-
-
-
3
-
-
-
6
-
-
3
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392552
Hisano
-
Microbial spermidine dehydroge ...
Citrobacter freundii, Flavobacterium dormitator, Pseudomonas aeruginosa, Serratia marcescens, Citrobacter freundii IFO12681, Pseudomonas aeruginosa IFO3080, Serratia marcescens IFO3046
J. Ferment. Bioeng.
69
335-340
1990
-
-
-
-
-
-
9
5
-
-
5
-
-
9
-
-
3
-
-
-
2
-
25
3
-
2
-
2
-
3
-
2
2
-
-
-
-
-
-
2
-
-
-
-
9
-
5
-
-
5
-
-
-
-
3
-
-
2
-
25
3
2
-
2
-
3
-
2
-
-
-
-
-
-
-
392550
Okada
Purification of spermidine deh ...
Serratia marcescens
Methods Enzymol.
94
303-305
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392548
Okada
Affinity chromatography of put ...
Serratia marcescens
J. Biochem.
85
1225-1233
1979
-
-
-
-
-
-
2
1
-
-
1
-
-
7
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392549
Okada
Substrate binding characterist ...
Serratia marcescens
J. Biochem.
85
1235-1243
1979
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
392545
Tabor
Biosynthesis and metabolism of ...
Serratia marcescens
Adv. Enzymol. Relat. Areas Mol. Biol.
36
203-268
1972
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
392547
Tabor
Spermidine dehydrogenase (Serr ...
Serratia marcescens
Methods Enzymol.
17B
746-753
1971
-
-
-
-
-
-
3
2
-
-
1
-
-
4
-
-
-
-
-
-
1
1
8
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
1
-
-
-
-
-
-
-
1
1
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
392546
Tabor
Identification of flavin adeni ...
Serratia marcescens
J. Biol. Chem.
245
5424-5433
1970
-
-
-
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
1
1
10
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
1
10
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-