Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methyl-L-glutamate + acceptor + H2O | Methylocella silvestris | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Methylocella silvestris BL2 | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylocella silvestris | - |
- |
- |
Methylocella silvestris BL2 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:monomethylamine-grown cell | - |
Methylocella silvestris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methyl-L-glutamate + acceptor + H2O | - |
Methylocella silvestris | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Methylocella silvestris BL2 | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O | the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN | Methylocella silvestris | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O | the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN | Methylocella silvestris BL2 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NMG dehydrogenase | - |
Methylocella silvestris |
NMGDH | - |
Methylocella silvestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Methylocella silvestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Methylocella silvestris |
Organism | Comment | Expression |
---|---|---|
Methylocella silvestris | the enzyme is induced by growth of the cells on monomethylamine, induction of an eight-gene cluster (Msil2632 to Msil2639) by monomethylamine and cotranscription as an operon | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the bacterium utilizes a methylated amino acid pathway gamma-glutamylmethylamide and N-methylglutamate for monomethylamine metabolism. The two key enzymes in this pathway are gamma-glutamylmethylamide synthetase and N-methylglutamate dehydrogenase. The gamma-glutamylmethylamide synthetase is essential for the pathway | Methylocella silvestris |