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Literature summary for 1.5.99.4 extracted from

  • Yu, W.; Wang, R.; Huang, H.; Xie, H.; Wang, S.
    Periplasmic nicotine dehydrogenase NdhAB utilizes pseudoazurin as its physiological electron acceptor in Agrobacterium tumefaciens S33 (2017), Appl. Environ. Microbiol., 83, e01050 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00087
-
 (S)-nicotine cosubstrate 2,6-dichlorophenolindophenol, pH 7, 30°C Agrobacterium tumefaciens
0.00124
-
 2,6-dichlorophenolindophenol pH 7, 30°C Agrobacterium tumefaciens
0.00167
-
 (S)-nicotine cosubstrate pseudoazurin, pH 7, 30°C Agrobacterium tumefaciens
0.00361
-
 pseudoazurin pH 7, 30°C Agrobacterium tumefaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Agrobacterium tumefaciens
-
-

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens S33
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purifiaction from the periplasmic fraction Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
 acceptor 2,6-dichlorophenolindophenol, pH 7, 30°C Agrobacterium tumefaciens
110
-
 acceptor pseudoazurin, pH 7, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
(S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens S33 (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
(S)-nicotine + pseudoazurin + H2O acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron Agrobacterium tumefaciens (S)-6-hydroxynicotine + reduced pseudoazurin
-
 ?
(S)-nicotine + pseudoazurin + H2O acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron Agrobacterium tumefaciens S33 (S)-6-hydroxynicotine + reduced pseudoazurin
-
 ?
additional information NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors Agrobacterium tumefaciens ?
-
-
additional information NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors Agrobacterium tumefaciens S33 ?
-
-

Synonyms

Synonyms Comment Organism
NdhAB
-
 Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 acceptor pseudoazurin Agrobacterium tumefaciens

General Information

General Information Comment Organism
physiological function disruption of the gene encoding pseudoazurin disables nicotine degradation Agrobacterium tumefaciens