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Literature summary for 1.5.99.12 extracted from

  • Tian, F.; Greplova, M.; Frebort, I.; Dale, N.; Napier, R.
    A highly selective biosensor with nanomolar sensitivity based on cytokinin dehydrogenase (2014), PLoS ONE, 9, e90877 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.99.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays Q9T0N8
-
-

Purification (Commentary)

Purification (Comment) Organism
octyl Sepharose column chromatography Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-zeatin + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
additional information adenosine, abscisic acid, gibberellic acid, and cis-zeatin riboside are no substrates, nor is the reduced dihydrozeatin. Aromatic cytokinins, kinetin and N6-benzyladenine, and the synthetic substituted urea cytokinin thidiazuron are also inactive, as are most cytokinin glucosides and the monophosphate N6-(DELTA2-isopentenyl)adenosine-5'-monophosphate Zea mays ?
-
 ?
N6-(DELTA2-isopentenyl)adenine + 2,6-dichlorophenolindophenol + H2O preferred substrate Zea mays ?
-
 ?
N6-(DELTA2-isopentenyl)adenosine + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
trans-zeatin + 2,6-dichlorophenolindophenol + H2O
-
 Zea mays ?
-
 ?
trans-zeatin riboside + 2,6-dichlorophenolindophenol + H2O most favorable substrate Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
CKX1
-
 Zea mays
N6-dimethylallyladenine dehydrogenase
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Zea mays