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Literature summary for 1.5.99.12 extracted from

  • Wang, Y.; Luo, J.P.; Wei, Z.J.; Zhang, J.C.
    Molecular cloning and expression analysis of a cytokinin oxidase (DhCKX) gene in Dendrobium huoshanense (2008), Mol. Biol. Rep., 36, 1331-1338.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information expression of the DhCKX gene is induced in roots, shoot apices and leaves, but not in stems, after treatment with 0.02 mM 6-benzylamino-purine Dendrobium huoshanense

Cloned(Commentary)

Cloned (Comment) Organism
-
Dendrobium huoshanense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60383
-
x * 60383, calculated from the deduced amino acid sequence Dendrobium huoshanense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Dendrobium huoshanense irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde ?
-
?
N6-isopentenyladenine + electron acceptor Dendrobium huoshanense
-
adenine + 3-methyl-2-butenal + reduced electron acceptor
-
ir
zeatin + electron acceptor Dendrobium huoshanense
-
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
-
ir

Organism

Organism UniProt Comment Textmining
Dendrobium huoshanense A2TLJ3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf low level Dendrobium huoshanense
-
additional information not detectable in stem Dendrobium huoshanense
-
root high level Dendrobium huoshanense
-
shoot high level in shoot apices Dendrobium huoshanense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde Dendrobium huoshanense ?
-
?
N6-isopentenyladenine + electron acceptor
-
Dendrobium huoshanense adenine + 3-methyl-2-butenal + reduced electron acceptor
-
ir
zeatin + electron acceptor
-
Dendrobium huoshanense 4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor
-
ir

Subunits

Subunits Comment Organism
? x * 60383, calculated from the deduced amino acid sequence Dendrobium huoshanense

Synonyms

Synonyms Comment Organism
CKX
-
Dendrobium huoshanense
DhCKX
-
Dendrobium huoshanense

Cofactor

Cofactor Comment Organism Structure
FAD
-
Dendrobium huoshanense

pI Value

Organism Comment pI Value Maximum pI Value
Dendrobium huoshanense calculated from the deduced amino acid sequence
-
6.6