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Literature summary for 1.5.99.12 extracted from

  • Kopecny, D.; Sebela, M.; Briozzo, P.; Spichal, L.; Houba-Herin, N.; Masek, V.; Joly, N.; Madzak, C.; Anzenbacher, P.; Laloue, M.
    Mechanism-based inhibitors of cytokinin oxidase/dehydrogenase attack FAD cofactor (2008), J. Mol. Biol., 380, 886-899.
    View publication on PubMed
Search for more literature for 1.5.99.12

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method in the presence of N6-(buta-2,3-dienyl)adenine or N6-(penta-2,3-dienyl)adenine Zea mays

Inhibitors

Inhibitors Comment Organism Structure
N6-(2-bromo-2-isopentenyl)adenine weak inhibition Zea mays
N6-(2-butynyl)adenine irreversible inhibition Zea mays
N6-(4-hydroxy-2-butynyl)adenine irreversible inhibition Zea mays
N6-(buta-2,3-dienyl)adenine strong irreversible mechanism based inhibition Zea mays
N6-(penta-2,3-dienyl)adenine strong irreversible mechanism based inhibition Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 N6-(2-isopentenyl)adenine 37°C Zea mays
0.092
-
 N6-(2-butynyl)adenine 37°C Zea mays
0.38
-
 N6-(4-hydroxy-2-butynyl)adenine 37°C Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69865
-
 1 * 69865, MALDI-TOF MS, crystal structure analysis Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Zea mays irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays Q9T0N8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
embryo strong expression in kernel Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo Zea mays ?
-
 ?
N6-(2-butynyl)adenine + 2,6-dichlorophenol indophenol very effective electron acceptor Zea mays ?
-
 ?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol very effective electron acceptor Zea mays ?
-
 ?
N6-(2-isopentenyl)adenosine + 2,6-dichlorophenol indophenol very effective electron acceptor Zea mays ?
-
 ?
N6-(4-hydroxy-2-butynyl)adenine + 2,6-dichlorophenol indophenol very effective electron acceptor Zea mays ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69865, MALDI-TOF MS, crystal structure analysis Zea mays

Synonyms

Synonyms Comment Organism
CKX
-
 Zea mays
cytokinin oxidase/dehydrogenase
-
 Zea mays
ZmCKO1
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 N6-(buta-2,3-dienyl)adenine
-
 Zea mays
0.026
-
 N6-(penta-2,3-dienyl)adenine
-
 Zea mays