Literature summary for 1.5.99.12 extracted from

Malito, E.; Coda, A.; Bilyeu, K.D.; Fraaije, M.W.; Mattevi, A.
Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis (2004), J. Mol. Biol., 341, 1237-1249.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Zea mays

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified free recombinant enzyme, or purified recombinant enzyme complexed with trans-zeatin, N6-isopentenyladenine, and N6-benzyladenine, sitting drop vapour diffusion method, 20°C, protein solution: 30 mg/ml protein, 20 mM HEPES-KOH, pH 7.5, mixed with equal volume of well solution containing 0.1 M sodium acetat, pH 4.6, 0.2 M ammonium sulfate, and 12% w/v PEG 5000 monomethylether, a few hours, soaking in ligand solution and/or cryoprotectant 30% glycerol before data collection, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, single isomorphous replacement, Fourier methods	Zea mays

Crystallization (Comment)

Organism

purified free recombinant enzyme, or purified recombinant enzyme complexed with trans-zeatin, N6-isopentenyladenine, and N6-benzyladenine, sitting drop vapour diffusion method, 20°C, protein solution: 30 mg/ml protein, 20 mM HEPES-KOH, pH 7.5, mixed with equal volume of well solution containing 0.1 M sodium acetat, pH 4.6, 0.2 M ammonium sulfate, and 12% w/v PEG 5000 monomethylether, a few hours, soaking in ligand solution and/or cryoprotectant 30% glycerol before data collection, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution, single isomorphous replacement, Fourier methods

Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Zea mays	enzyme has a major role in control of cytokinin plant hormone levels	?	-	?
N6-isopentenyladenine + FAD + H2O	Zea mays	oxidative degradation	adenine + 3-methylbut-2-enal + FADH2	-	ir

Organism

Organism	UniProt	Comment	Textmining
Zea mays	Q9T0N8	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor	reaction scheme and mechanism, the substrate displays a plug-into-socket binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin, bipartite architecture of the catalytic centre, which consists of a funnel-shaped region on the protein surface and an internal cavity lined by the flavin ring, the 3 active site regions are connected by a pore of about 4 A diameter	Zea mays	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	enzyme has a major role in control of cytokinin plant hormone levels	Zea mays	?	-	?
additional information	the substrate binding site is located at amino acid residues 245-491	Zea mays	?	-	?
N6-benzyladenine + FAD + H2O	substrate binding mechanism and structure, overview	Zea mays	adenine + ? + FADH2	-	ir
N6-isopentenyladenine + FAD + H2O	oxidative degradation	Zea mays	adenine + 3-methylbut-2-enal + FADH2	-	ir
N6-isopentenyladenine + FAD + H2O	substrate binding mechanism and structure, overview	Zea mays	adenine + 3-methylbut-2-enal + FADH2	-	ir
trans-zeatin + FAD + H2O	substrate binding mechanism and structure, overview	Zea mays	? + FADH2	-	ir

Synonyms

Synonyms	Comment	Organism
CKX	-	Zea mays

Cofactor

Cofactor	Comment	Organism	Structure
FAD	binding site structure comprising amino acid residues 40-244 and 492-534	Zea mays