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Literature summary for 1.5.98.3 extracted from
- Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Go1 (1998), J. Bacteriol., 180, 2027-2032 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
2-hydroxyphenazine | at pH 7.0 and 25°C | Methanosarcina mazei |  |
| 0.25 | - |
phenazine | at pH 7.0 and 25°C | Methanosarcina mazei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Methanosarcina mazei | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced coenzyme F420 + methanophenazine | Methanosarcina mazei | - |
oxidized coenzyme F420 + dihydromethanophenazine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
with phenazine as cosubstrate, at pH 7.0 and 25°C | Methanosarcina mazei |
| 4.6 | - |
with phenazine-1-carboxylic acid as cosubstrate, at pH 7.0 and 25°C | Methanosarcina mazei |
| 8.4 | - |
with 2-bromophenazine as cosubstrate, at pH 7.0 and 25°C | Methanosarcina mazei |
| 8.8 | - |
with 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 25°C | Methanosarcina mazei |
| 10 | - |
with methyl viologen as cosubstrate, at pH 7.0 and 25°C | Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced coenzyme F420 + 2-bromophenazine | - |
Methanosarcina mazei | ? | - |
? | |
| reduced coenzyme F420 + 2-hydroxyphenazine | - |
Methanosarcina mazei | ? | - |
? | |
| reduced coenzyme F420 + methanophenazine | - |
Methanosarcina mazei | oxidized coenzyme F420 + dihydromethanophenazine | - |
? | |
| reduced coenzyme F420 + methyl viologen | - |
Methanosarcina mazei | ? | - |
? | |
| reduced coenzyme F420 + phenazine | - |
Methanosarcina mazei | ? | - |
? | |
| reduced coenzyme F420 + phenazine-1-carboxylic acid | - |
Methanosarcina mazei | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| F420H2 dehydrogenase | - |
Methanosarcina mazei |
| fpoBCDIF | - |
Methanosarcina mazei |
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