General Stability | Organism |
---|---|
the purified enzyme becomes rapidly inactivated by repeated freezing and thawing | Methanolobus tindarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
reduced coenzyme F420 | at pH 6.8 and 37°C | Methanolobus tindarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methanolobus tindarius | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 16 mol non-heme iron per mol enzyme | Methanolobus tindarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Methanolobus tindarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanolobus tindarius | - |
- |
- |
Methanolobus tindarius DSM 2278 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography | Methanolobus tindarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced coenzyme F420 + oxidized metronidazole | - |
Methanolobus tindarius | oxidized coenzyme F420 + reduced metronidazole | - |
? | |
reduced coenzyme F420 + oxidized metronidazole | - |
Methanolobus tindarius DSM 2278 | oxidized coenzyme F420 + reduced metronidazole | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE | Methanolobus tindarius |
Synonyms | Comment | Organism |
---|---|---|
F420H2-dehydrogenase | - |
Methanolobus tindarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Methanolobus tindarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.5 | - |
reduced coenzyme F420 | at pH 6.8 and 37°C | Methanolobus tindarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Methanolobus tindarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanolobus tindarius | isoelectric focusing | - |
5 |