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Literature summary for 1.5.98.1 extracted from

  • Kojima, H.; Moll, J.; Kahnt, J.; Fukui, M.; Shima, S.
    A reversed genetic approach reveals the coenzyme specificity and other catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate (2014), Environ. Microbiol., 16, 3431-3442.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured archaeon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
oxidized coenzyme F420 pH 6.0, 20°C uncultured archaeon
0.005
-
5,10-methylenetetrahydromethanopterin pH 6.0, 20°C uncultured archaeon
0.015
-
5,10-methylenetetrahydromethanopterin pH 6.0, 20°C uncultured archaeon

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30283
-
x * 30283, calculated uncultured archaeon
30287
-
x * 30287, calculated from sequence uncultured archaeon

Organism

Organism UniProt Comment Textmining
uncultured archaeon D1JH91
-
-

Purification (Commentary)

Purification (Comment) Organism
-
uncultured archaeon

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1100
-
pH 6.0, 20°C uncultured archaeon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
uncultured archaeon 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
? x * 30283, calculated uncultured archaeon
? x * 30287, calculated from sequence uncultured archaeon

Synonyms

Synonyms Comment Organism
CBH37899
-
uncultured archaeon
F420-dependent methylenetetrahydromethanopterin dehydrogenase
-
uncultured archaeon
MTD
-
uncultured archaeon

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
uncultured archaeon

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 55% of maximal activity, 80°C: about 75% of maximal activity uncultured archaeon

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
enzyme diluted in 120 mM potassium phosphate buffer pH 6.0 at temperature below 10°C shows a half-life of less than 1 h. Higher concentrations of potassium phosphate stabilize, the optimum concentration being 0.5-0.8 M uncultured archaeon

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at uncultured archaeon

Cofactor

Cofactor Comment Organism Structure
additional information when NAD+ or NADP+ is used instead of F420, the specific activity is less than 0.001 U/mg uncultured archaeon

pI Value

Organism Comment pI Value Maximum pI Value
uncultured archaeon calculated from sequence
-
6.1