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Literature summary for 1.5.98.1 extracted from

  • Mukhopadhyay, B.; Daniels, L.
    Aerobic purification of N5,N10-methylenetetrahydromethanopterin dehydrogenase, separated from N5,N10-methenyltetrahydromethanopterin cyclohydrolase, from Methanobacterium thermoautotrophicum strain Marburg (1989), Can. J. Microbiol., 35, 499-507.
    View publication on PubMed

General Stability

General Stability Organism
stable in phosphate buffer with or without glycerol or ammonium sulfate under both aerobic and anaerobic conditions Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable under air Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
197
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 absolutely dependent on coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 required Methanothermobacter thermautotrophicus