BRENDA - Enzyme Database show
show all sequences of 1.5.8.3

Cloning and mapping of the cDNA for human sarcosine dehydrogenase, a flavoenzyme defective in patients with sarcosinemia

Eschenbrenner, M.; Schuman Jorns, M.; Genomics 59, 300-308 (1999)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
sarcosine dehydrogenase is defective in patient with sarcosinemia
Homo sapiens
Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99505
-
1 * 99505
Homo sapiens
99510
-
including covalently bound FAD
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sarcosine + acceptor + H2O
Homo sapiens
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
glycine + formaldehyde + reduced acceptor
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
adult and fetal
Homo sapiens
-
liver
adult and fetal
Homo sapiens
-
pancreas
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sarcosine + acceptor + H2O
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
392509
Homo sapiens
glycine + formaldehyde + reduced acceptor
-
-
-
?
sarcosine + acceptor + H2O
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
392509
Homo sapiens
glycine + formaldehyde + reduced acceptor
-
392509
Homo sapiens
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 99505
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
covalent bound FAD linked via 8a-position of the isoalloxazin ring to the imidazole N(3) of a histidine residue, structural homology between the amino acid sequence of the flavin-peptides
Homo sapiens
tetrahydrofolate
-
Homo sapiens
Application (protein specific)
Application
Commentary
Organism
medicine
sarcosine dehydrogenase is defective in patient with sarcosinemia
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
covalent bound FAD linked via 8a-position of the isoalloxazin ring to the imidazole N(3) of a histidine residue, structural homology between the amino acid sequence of the flavin-peptides
Homo sapiens
tetrahydrofolate
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99505
-
1 * 99505
Homo sapiens
99510
-
including covalently bound FAD
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sarcosine + acceptor + H2O
Homo sapiens
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
glycine + formaldehyde + reduced acceptor
-
Homo sapiens
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
adult and fetal
Homo sapiens
-
liver
adult and fetal
Homo sapiens
-
pancreas
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sarcosine + acceptor + H2O
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
392509
Homo sapiens
glycine + formaldehyde + reduced acceptor
-
-
-
?
sarcosine + acceptor + H2O
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate
392509
Homo sapiens
glycine + formaldehyde + reduced acceptor
-
392509
Homo sapiens
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 99505
Homo sapiens
Other publictions for EC 1.5.8.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742694
Kim
-
Expression of sarcosine-metab ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
9
7132-7139
2016
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743612
Heger
Sarcosine up-regulates expres ...
Homo sapiens
PLoS ONE
11
e0165830
2016
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742459
Umair
Sarcosine metabolism in Haemo ...
no activity in Haemonchus contortus, no activity in Teladorsagia circumcincta
Exp. Parasitol.
134
1-6
2013
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743719
Green
TMEFF2 and SARDH cooperate to ...
Homo sapiens
Prostate
73
1561-1575
2013
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725421
Chen
The tumor suppressor activity ...
Homo sapiens
J. Biol. Chem.
286
16091-16100
2011
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655081
Kato
Enzymatic activity and stabili ...
Pseudomonas putida
Biotechnol. Bioeng.
84
415-423
2003
1
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1
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392510
Tang
Purification and some properti ...
Pseudomonas putida, Pseudomonas putida RS65
Food Sci. Agric. Chem.
3
36-41
2001
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2
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392509
Eschenbrenner
Cloning and mapping of the cDN ...
Homo sapiens
Genomics
59
300-308
1999
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392511
Bergeron
Molecular cloning and tissue d ...
Rattus norvegicus
Eur. J. Biochem.
257
556-561
1998
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390333
Smith
Osmotic control of glycine bet ...
Sinorhizobium meliloti
J. Bacteriol.
170
3142-3149
1988
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392494
Cook
Dimethylglycine dehydrogenase ...
Rattus norvegicus
Methods Enzymol.
122
255-260
1986
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289023
Yamada
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A novel metabolic pathway for ...
Pseudomonas putida
FEMS Microbiol. Lett.
30
337-340
1985
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392496
Porter
Enzymatic properties of dimeth ...
Rattus norvegicus
Arch. Biochem. Biophys.
243
396-407
1985
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392497
Cook
The amino acid sequences of th ...
Rattus norvegicus
J. Biol. Chem.
260
12998-13002
1985
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392498
Steenkamp
The effect of tetrahydrofolate ...
Sus scrofa
Biochem. J.
203
707-715
1982
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392499
Wittwer
Identification of the folate-b ...
Rattus norvegicus
J. Biol. Chem.
256
4109-4115
1981
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392500
Wittwer
Identification of the folate-b ...
Rattus norvegicus
J. Biol. Chem.
256
4102-4108
1981
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392501
Cook
Identification of the covalent ...
Rattus norvegicus
J. Biol. Chem.
259
12475-12480
1980
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392502
Oka
-
Sarcosine dehydrogenase from P ...
Corynebacterium sp., Pseudomonas putida
Agric. Biol. Chem.
43
1197-1203
1979
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389889
Bater
The characterisation of induci ...
Pseudomonas aeruginosa, Pseudomonas reptilivorous
Biochim. Biophys. Acta
468
209-226
1977
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172080
Tsuru
-
Creatinine decomposing enzymes ...
Pseudomonas putida
Agric. Biol. Chem.
40
1011-1018
1976
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392505
Pinto
Characterization of the peptid ...
Pseudomonas sp.
Arch. Biochem. Biophys.
169
483-491
1975
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392506
Patek
Purification and characterizat ...
Rattus norvegicus
Arch. Biochem. Biophys.
150
347-354
1972
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392507
Frisell
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Sarcosine dehydrogenase and di ...
Macaca mulatta, Rattus norvegicus
Methods Enzymol.
17A
976-981
1970
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392508
Frisell
Separation and purification of ...
Rattus norvegicus
J. Biol. Chem.
237
94-98
1962
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