Application | Comment | Organism |
---|---|---|
nutrition | quality control, development of an amperometric enzyme electrode sensor for rapid detection of trimethylamine in different fish muscle samples using phenazine methosulfate as redox cofactor and NBT-based colorimetric detectiona nd quantification | Methylophilus methylotrophus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | - |
Methylophilus methylotrophus | |
ferricinium | Val334 is involved in binding and initiates a conformational change interrupting the electron transfer between FMN and the [4Fe-4S] cluster | Methylophilus methylotrophus | |
additional information | no inhibition by (dimethylamino)methylene ferrocene, ferrocenedicarboxylic acid, ferrocenemonocarboxylic acid, ferrocenedimathanol, (dimethylamino)methylene ferrocene, ferricyanide, and thionine | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism, steady-state kinetics using (dimethylamino)methylene ferrocene, pH-dependency and effects on kinetics, overview | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme contains a [4Fe-4S] cluster, electron transfer between FMN and the [4Fe-4S] cluster | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + FMN | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + FMNH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein | redox mediation mechanism, overview | Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recycling of the prosthetic electron-transferring group FMN by ferricinium, ferrocenedicarboxylic acid, ferrocenemonocarboxylic acid, ferrocenedimathanol, (dimethylamino)methylene ferrocene, and with lower activity by ferricyanide, no electron-transfer activity with 2,6-dichlorophenolindophenol, and thionine | Methylophilus methylotrophus | ? | - |
? | |
trimethylamine + H2O + FMN | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + FMNH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TMADH | - |
Methylophilus methylotrophus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methylophilus methylotrophus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methylophilus methylotrophus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Methylophilus methylotrophus |