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Literature summary for 1.5.8.2 extracted from
- Reductive half-reaction of the H172Q mutant of trimethylamine dehydrogenase: Evidence against a carbanion mechanism and assignment of kinetically influential ionizations in the enzyme-substrate complex (1999), Biochem. J., 341, 307-314.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H172Q | no change of turnover, but 25fold increase of Km-value | Methylophilus methylotrophus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| trimethylamine | - |
Methylophilus methylotrophus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
| trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus W3A1 | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus methylotrophus | - |
- |
- |
| Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Methylophilus methylotrophus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
| trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Methylophilus methylotrophus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
trimethylamine | - |
Methylophilus methylotrophus | |
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Release 2023.1 (January 2023)
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