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Literature summary for 1.5.8.2 extracted from

  • Ertrughrul, O.W.D.; Errington, N.; Raza, S.; Sutcliffe, M.J.; Rowe, A.J.; Scrutton, N.S.
    Probing the stabilizing role of C-terminal residues in trimethylamine dehydrogenase (1998), Protein Eng., 11, 447-455.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylophilus methylotrophus

Protein Variants

Protein Variants Comment Organism
C-terminal truncations C-terminal truncations of 5, 10 or 17 residues do not affect dimer stability, but compromise the ability of the enzyme to become covalently coupled with FMN in the active site Methylophilus methylotrophus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
trimethylamine mutant DELTA725-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
0.0047
-
trimethylamine mutant DELTA720-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
0.0096
-
trimethylamine recombinant wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
0.0137
-
trimethylamine native wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 4Fe-4S-center Methylophilus methylotrophus
Fe 4.5 mol iron per mol enzyme Methylophilus methylotrophus

Organism

Organism UniProt Comment Textmining
Methylophilus methylotrophus
-
-
-
Methylophilus methylotrophus W3A1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Methylophilus methylotrophus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Methylophilus methylotrophus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
Methylophilus methylotrophus dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
trimethylamine + H2O + 2,6-dichlorophenolindophenol
-
Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
?
trimethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus dimethylamine + formaldehyde + reduced phenazine methosulfate
-
?
trimethylamine + H2O + phenazine methosulfate
-
Methylophilus methylotrophus W3A1 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
trimethylamine mutant delta725-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
6.4
-
trimethylamine mutant delta720-729, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
10.2
-
trimethylamine recombinant wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus
15.6
-
trimethylamine native wild-type form, electron acceptor: phenazine methosulfate Methylophilus methylotrophus

Cofactor

Cofactor Comment Organism Structure
ADP
-
Methylophilus methylotrophus
FMN
-
Methylophilus methylotrophus