Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli DH5alpha | Methanosarcina mazei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
reduced ferredoxin | pH 7.0, temperature not specified in the publication | Methanosarcina mazei | |
0.002 | - |
oxidized coenzyme F420 | pH 7.0, temperature not specified in the publication | Methanosarcina mazei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | as a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei | 5737 | - |
membrane | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | presence of two FeS clusters, 8.7 mol of nonheme iron and 5.1 mol of acid-labile sulfur per mol of protein | Methanosarcina mazei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | Methanosarcina mazei | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | Methanosarcina mazei DSM 3647 | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
reduced coenzyme F420 + 2 oxidized ferredoxin | Methanosarcina mazei | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r | |
reduced coenzyme F420 + 2 oxidized ferredoxin | Methanosarcina mazei DSM 3647 | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | Q8PZ67 | - |
- |
Methanosarcina mazei DSM 3647 | Q8PZ67 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina mazei | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
Methanosarcina mazei DSM 3647 | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex | Methanosarcina mazei DSM 3647 | reduced coenzyme F420 + 2 oxidized ferredoxin | - |
r | |
reduced coenzyme F420 + 2 oxidized ferredoxin | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r | |
reduced coenzyme F420 + 2 oxidized ferredoxin | when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex | Methanosarcina mazei DSM 3647 | oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Fd:F420 oxidoreductase | - |
Methanosarcina mazei |
FpoF protein | - |
Methanosarcina mazei |
mm0627 | - |
Methanosarcina mazei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanosarcina mazei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | presence of 1.0 mol FAD per mol protein | Methanosarcina mazei |