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Literature summary for 1.5.7.1 extracted from

  • Clark, J.E.; Ljungdahl, L.G.
    Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum (1984), J. Biol. Chem., 259, 10845-10849.
    View publication on PubMed
Search for more literature for 1.5.7.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 5-methyltetrahydrofolate
-
 Clostridium formicaceticum
11.1
-
 benzyl viologen
-
 Clostridium formicaceticum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur protein, contains 15.2 iron and 19.5 acid labile sulfide per 237000 Da enzyme Clostridium formicaceticum
Zinc enzyme contains 2.3 zinc per 237000 Da enzyme Clostridium formicaceticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE Clostridium formicaceticum
35000
-
 alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE Clostridium formicaceticum
237000
-
 gel filtration Clostridium formicaceticum

Organism

Organism UniProt Comment Textmining
Clostridium formicaceticum
-
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme loses 50% of the activity within 2 h in aerobic buffer (0.1 M Tris/HCl buffer, pH 7.4) with or without a reducing agent and in anaerobic buffer without a reducing agent Clostridium formicaceticum

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium formicaceticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
139
-
-
 Clostridium formicaceticum

Storage Stability

Storage Stability Organism
10°C, pH 7.4, 50 mM Tris/HCl containing 20% glycerol and 2 mM dithionite in an anaerobic chamber Clostridium formicaceticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + FADH2
-
 Clostridium formicaceticum 5,10-methylenetetrahydrofolate + FAD
-
 ?
5,10-methylenetetrahydrofolate + reduced ferredoxin
-
 Clostridium formicaceticum 5-methyltetrahydrofolate + ferredoxin
-
 ?
5-methyltetrahydrofolate + benzyl viologen
-
 Clostridium formicaceticum 5,10-methylenetetrahydrofolate + reduced benzyl viologen
-
 ?
5-methyltetrahydrofolate + benzyl viologen 11% of the activity with methylene blue Clostridium formicaceticum 5,10-methylenetetrahydrofolate + reduced benzyl viologen
-
 ?
5-methyltetrahydrofolate + FAD 11% of the activity with methylene blue Clostridium formicaceticum 5,10-methylenetetrahydrofolate + FADH2
-
 ?
5-methyltetrahydrofolate + menadione 25% of the activity with methylene blue Clostridium formicaceticum 5,10-methylenetetrahydrofolate + reduced menadiol
-
 ?
5-methyltetrahydrofolate + rubredoxin 11% of the activity with methylene blue Clostridium formicaceticum 5,10-methylenetetrahydrofolate + reduced rubredoxin
-
 ?
additional information no activity with pyridine nucleotides Clostridium formicaceticum ?
-
 ?

Subunits

Subunits Comment Organism
octamer alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE Clostridium formicaceticum

Synonyms

Synonyms Comment Organism
5,10-methylenetetrahydrofolate reductase
-
 Clostridium formicaceticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
 Clostridium formicaceticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.1 7.6 the enzyme is more stable in Tris/HCl than in triethanolamine/HCl or phosphate buffer Clostridium formicaceticum

Cofactor

Cofactor Comment Organism Structure
FAD contains 1.7 FAD per 237000 Da enzyme Clostridium formicaceticum