BRENDA - Enzyme Database
show all sequences of 1.5.5.2

A conserved active site tyrosine residue of proline dehydrogenase helps enforce the preference for proline over hydroxyproline as the substrate

Ostrander, E.L.; Larson, J.D.; Schuermann, J.P.; Tanner, J.J.; Biochemistry 48, 951-959 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
mutant Y540S
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
Y540A
a construct consisting of residue 86-630 of PutA is used
Escherichia coli
Y540S
a construct consisting of residue 86-630 of PutA is used
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-tetrahydro-2-furoic acid
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
20
L-proline
Y540A mutant
Escherichia coli
59
-
L-proline
wild type
Escherichia coli
100
-
L-proline
Y540S mutant
Escherichia coli
190
-
trans-4-hydroxy-L-proline
Y540S mutant
Escherichia coli
310
-
trans-4-hydroxy-L-proline
Y540A mutant
Escherichia coli
750
-
trans-4-hydroxy-L-proline
wild type
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P09546
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-proline + oxidized dichlorophenolindophenol
-
696361
Escherichia coli
1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
-
-
?
trans-4-hydroxy-L-proline + oxidized dichlorophenolindophenol
-
696361
Escherichia coli
?
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
trans-4-hydroxy-L-proline
Y540S mutant
Escherichia coli
2.5
-
trans-4-hydroxy-L-proline
wild type
Escherichia coli
2.9
-
trans-4-hydroxy-L-proline
Y540A mutant
Escherichia coli
7.8
-
L-proline
Y540S mutant
Escherichia coli
13
-
L-proline
Y540A mutant
Escherichia coli
29
-
L-proline
wild type
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.084
-
L-tetrahydro-2-furoic acid
-
Escherichia coli
1.5
-
L-tetrahydro-2-furoic acid
mutant Y540S
Escherichia coli
7.9
-
L-tetrahydro-2-furoic acid
mutant Y540A
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
mutant Y540S
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Y540A
a construct consisting of residue 86-630 of PutA is used
Escherichia coli
Y540S
a construct consisting of residue 86-630 of PutA is used
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-tetrahydro-2-furoic acid
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.084
-
L-tetrahydro-2-furoic acid
-
Escherichia coli
1.5
-
L-tetrahydro-2-furoic acid
mutant Y540S
Escherichia coli
7.9
-
L-tetrahydro-2-furoic acid
mutant Y540A
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5
20
L-proline
Y540A mutant
Escherichia coli
59
-
L-proline
wild type
Escherichia coli
100
-
L-proline
Y540S mutant
Escherichia coli
190
-
trans-4-hydroxy-L-proline
Y540S mutant
Escherichia coli
310
-
trans-4-hydroxy-L-proline
Y540A mutant
Escherichia coli
750
-
trans-4-hydroxy-L-proline
wild type
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-proline + oxidized dichlorophenolindophenol
-
696361
Escherichia coli
1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
-
-
?
trans-4-hydroxy-L-proline + oxidized dichlorophenolindophenol
-
696361
Escherichia coli
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
trans-4-hydroxy-L-proline
Y540S mutant
Escherichia coli
2.5
-
trans-4-hydroxy-L-proline
wild type
Escherichia coli
2.9
-
trans-4-hydroxy-L-proline
Y540A mutant
Escherichia coli
7.8
-
L-proline
Y540S mutant
Escherichia coli
13
-
L-proline
Y540A mutant
Escherichia coli
29
-
L-proline
wild type
Escherichia coli
Other publictions for EC 1.5.5.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743834
Huijbers
Proline dehydrogenase from Th ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Sci. Rep.
7
43880
2017
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-
1
1
1
-
-
3
-
-
-
2
-
6
-
-
-
-
-
-
4
-
4
1
1
-
-
3
1
-
-
3
-
-
-
-
-
1
3
1
1
-
-
-
-
3
-
-
-
2
-
-
-
-
-
-
4
-
4
1
1
-
-
3
1
-
-
-
-
-
-
-
3
3
741588
Hancock
Co-regulation of mitochondria ...
Homo sapiens, Mus musculus
Amino Acids
48
859-872
2016
-
-
2
-
1
-
12
-
2
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
1
-
-
12
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
6
6
-
-
-
741858
Cabassa-Hourton
Proteomic and functional anal ...
Arabidopsis thaliana
Biochem. J.
473
2623-2634
2016
-
-
-
-
1
-
-
-
3
-
-
-
-
5
-
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1
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1
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3
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1
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-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
741642
Wang
Molecular cloning and express ...
Jatropha curcas
Appl. Biochem. Biotechnol.
175
2413-2426
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
6
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
2
-
-
-
-
-
-
-
1
1
2
2
1
-
-
742162
Huijbers
High yields of active Thermus ...
Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
Biotechnol. J.
10
395-403
2015
-
1
1
-
-
-
1
-
1
-
3
2
-
5
-
-
1
-
-
-
1
-
6
1
1
-
2
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
1
-
3
2
-
-
-
1
-
-
1
-
6
1
1
-
2
-
1
-
-
-
-
1
1
-
-
-
740734
Moxley
Evidence for hysteretic substr ...
Escherichia coli
J. Biol. Chem.
289
3639-3651
2014
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
3
3
742487
Schertl
Biochemical characterization ...
Arabidopsis thaliana
FEBS J.
281
2794-2804
2014
-
-
-
-
-
-
2
-
2
-
-
1
-
4
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
724389
Serrano
Kinetic and isotopic character ...
Mycobacterium tuberculosis
Biochemistry
52
5009-5015
2013
-
-
-
-
2
-
-
3
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
741732
Kawakami
Comparative analysis of the c ...
Pyrococcus furiosus, Pyrococcus horikoshii, Pyrococcus horikoshii OT-3, Thermococcus kodakarensis, Thermococcus kodakarensis KOD1 JCM12380, Thermococcus profundus, Thermococcus profundus DSM 9503
Appl. Microbiol. Biotechnol.
97
3419-3427
2013
-
-
4
-
-
-
8
-
-
-
-
-
-
13
-
-
4
-
-
-
-
-
22
7
4
-
4
-
4
-
-
4
-
-
-
-
-
7
7
-
-
-
-
14
-
-
-
-
-
-
-
-
-
7
-
-
-
-
22
7
7
-
7
-
7
-
-
-
-
8
14
-
-
-
742641
Omidinia
Expression, purification and ...
Pseudomonas putida, Pseudomonas putida POS-F84
Indian J. Microbiol.
53
297-302
2013
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
2
2
1
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
2
1
1
-
-
1
1
-
-
-
1
1
-
-
-
743635
Paes
Proline dehydrogenase regulat ...
Trypanosoma cruzi, Trypanosoma cruzi CL Brener
PLoS ONE
8
e69419
2013
-
-
1
-
-
-
-
1
1
-
-
2
-
5
-
-
1
-
-
1
-
-
10
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
1
-
-
2
-
-
-
1
-
1
-
-
10
1
1
-
-
-
1
-
-
-
1
2
2
1
-
-
724345
Moxley
Rapid reaction kinetics of pro ...
Escherichia coli
Biochemistry
51
511-520
2012
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
739932
Kawakami
L-proline dehydrogenases in hy ...
Pyrococcus horikoshii
Appl. Microbiol. Biotechnol.
93
83-93
2012
-
-
-
-
-
-
-
1
-
-
3
1
-
5
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
3
1
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
711252
Srivastava
The structure of the proline u ...
Escherichia coli
Biochemistry
49
560-569
2010
-
-
1
1
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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-
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-
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1
-
1
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1
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
696361
Ostrander
A conserved active site tyrosi ...
Escherichia coli
Biochemistry
48
951-959
2009
-
-
1
1
2
-
1
6
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
-
-
1
3
-
-
-
-
1
1
1
2
-
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1
3
6
-
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1
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2
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6
-
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-
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-
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-
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-
671977
Zhu
Exploring the proline-dependen ...
Escherichia coli
Biochemistry
44
12297-12306
2005
-
-
-
-
4
-
-
4
1
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
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-
-
4
-
-
-
-
4
1
-
-
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-
1
-
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-
-
4
-
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-
658033
Zhang
Structures of the Escherichia ...
Escherichia coli
Biochemistry
43
12539-12548
2004
-
-
1
1
1
-
3
2
-
-
1
1
-
3
-
-
1
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
3
-
-
-
-
1
-
1
1
-
-
3
3
2
-
-
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
658211
Baban
Probing a hydrogen bond pair a ...
Escherichia coli
Biochim. Biophys. Acta
1701
49-59
2004
-
-
1
-
3
-
-
4
-
-
-
-
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2
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1
-
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4
-
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-
-
-
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-
1
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
658668
Kawakami
Gene and primary structures of ...
Thermococcus profundus
Extremophiles
8
99-108
2004
-
-
1
-
-
-
-
1
-
1
5
-
-
10
-
-
1
-
-
-
1
-
2
1
1
-
1
-
1
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
5
-
-
-
-
1
-
-
1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
659987
Lee
Structure of the proline dehyd ...
Escherichia coli
Nat. Struct. Biol.
10
109-114
2003
-
-
-
1
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-
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-
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1
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3
-
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1
1
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1
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1
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1
1
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-
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-
-
392565
Nadaraia
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
57
1925-1927
2001
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392570
Sakuraba
Purification, characterization ...
Thermococcus profundus
Appl. Environ. Microbiol.
67
1470-1475
2001
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392572
Chaudhary
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Proline dehydrogenase activity ...
Vigna radiata
Indian J. Exp. Biol.
37
1234-1240
1999
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392571
Brown
Conformational change and memb ...
Escherichia coli
J. Biol. Chem.
268
8972-8979
1993
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392569
Deutch
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Oxidation of L-thiazolidine-4- ...
Escherichia coli
J. Gen. Microbiol.
138
1593-1598
1992
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722591
Brown
Redesigned purification yields ...
Escherichia coli
J. Biol. Chem.
267
13086-13092
1992
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392555
Wood
Membrane association of prolin ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
84
373-377
1987
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392556
Graham
Proline dehydrogenase from Esc ...
Escherichia coli
J. Biol. Chem.
259
2656-2661
1984
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392560
Scarpulla
Membrane-bound proline dehydro ...
Escherichia coli
J. Biol. Chem.
253
5997-6001
1978
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