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Literature summary for 1.5.5.1 extracted from

  • Usselman, R.J.; Fielding, A.J.; Frerman, F.E.; Watmough, N.J.; Eaton, G.R.; Eaton, S.S.
    Impact of mutations on the midpoint potential of the [4Fe-4S]+1,+2 cluster and on catalytic activity in electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO) (2008), Biochemistry, 47, 92-100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43 cells Cereibacter sphaeroides

Protein Variants

Protein Variants Comment Organism
T525A the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides
Y501F the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides
Y501F/T525A the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose colmn chromatography and Source 15Q column chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced electron-transferring flavoprotein + ubiquinone-2
-
Cereibacter sphaeroides electron-transferring flavoprotein + ubiquinol-2
-
?

Synonyms

Synonyms Comment Organism
electron transfer flavoprotein-ubiquinone oxidoreductase
-
Cereibacter sphaeroides
ETF-QO
-
Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center iron-sulfur flavoprotein that contains a single [4Fe-4S]2+,1+ Cereibacter sphaeroides
FAD contains one FAD Cereibacter sphaeroides