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Literature summary for 1.5.5.1 extracted from
- Alternative quinone substrates and inhibitors of human electron-transfer flavoprotein-ubiquinone oxidoreductase (2004), Biochem. J., 378, 633-640.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in insect Sf9 cells via transfection using the baculovirus system | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-(3-methylpentyl)-4,6-dinitrophenol | noncompetitive | Homo sapiens |  |
| 2-n-heptyl-4-hydroxyquinoline N-oxide | - |
Homo sapiens | |
| additional information | inhibition mechanism | Homo sapiens | |
| Pentachlorophenol | noncompetitive | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | substrate binding and steady-state reaction kinetics | Homo sapiens | |
| 0.0023 | - |
pentadecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0069 | - |
6-bis(isoprenyl)ubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0073 | - |
nonylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0079 | - |
decylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0082 | - |
heptylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0089 | - |
bromodecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0095 | - |
6-isoprenylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0099 | - |
hydroxydecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0163 | - |
menadione | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0195 | - |
duroquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0265 | - |
tridecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | enzyme contains iron-sulfur clusters | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| reduced electron-transferring-flavoprotein + ubiquinone | Homo sapiens | ETF is the intermediate electron carrier between dehydrogenases and the enzyme, enzyme mediates between eleven mitochondrial proteins and the ubiquinone pool | electron-transferring-flavoprotein + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Sf9 insect cells | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | specificity for different ubiquinone derivatives, overview | Homo sapiens | ? | - |
? | |
| reduced electron-transferring-flavoprotein + 2,5-dibromo-3-methyl-6-isopropyl-4-benzoquinone | - |
Homo sapiens | electron-transferring-flavoprotein + 2,5-dibromo-3-methyl-6-isopropyl-4-benzoquinol | - |
? | |
| reduced electron-transferring-flavoprotein + 6-bis(isoprenyl)ubiquinone | optimal ubiquinone derivative | Homo sapiens | electron-transferring-flavoprotein + 6-bis(isoprenyl)ubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + 6-isoprenylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + 6-isoprenylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + bromodecylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + bromodecylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + decylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + decylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + duroquinone | - |
Homo sapiens | electron-transferring-flavoprotein + duroquinol | - |
? | |
| reduced electron-transferring-flavoprotein + heptylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + heptylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + hydroxydecylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + hydroxydecylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + menadione | - |
Homo sapiens | electron-transferring-flavoprotein + menadiol | - |
? | |
| reduced electron-transferring-flavoprotein + nonylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + nonylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + pentadecylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + pentadecylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + tridecylubiquinone | - |
Homo sapiens | electron-transferring-flavoprotein + tridecylubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + ubiquinone | ETF is the intermediate electron carrier between dehydrogenases and the enzyme, enzyme mediates between eleven mitochondrial proteins and the ubiquinone pool | Homo sapiens | electron-transferring-flavoprotein + ubiquinol | - |
? | |
| reduced electron-transferring-flavoprotein + ubiquinone | ubiquinone is 2,3-dimethoxy-5-methyl-1,4-benzoquinone | Homo sapiens | electron-transferring-flavoprotein + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| electron-transfer flavoprotein-2,3-dimethoxy-5-methyl-1,4-benzoquinone oxidoreductase | - |
Homo sapiens |
| electron-transfer flavoprotein-ubiquinone oxidoreductase | - |
Homo sapiens |
| ETF-QO | - |
Homo sapiens |
| ETF-ubiquinone oxidoreductase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.6 | - |
menadione | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 7.4 | - |
pentadecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 18.1 | - |
duroquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 31.5 | - |
tridecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 34.5 | - |
6-isoprenylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 54 | - |
heptylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 59.9 | - |
decylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 60.8 | - |
bromodecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 61.3 | - |
nonylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 67 | - |
hydroxydecylubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 68.7 | - |
6-bis(isoprenyl)ubiquinone | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
2-(3-methylpentyl)-4,6-dinitrophenol | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
| 0.0077 | - |
Pentachlorophenol | recombinant enzyme, pH 7.4, 25°C | Homo sapiens | |
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