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Literature summary for 1.5.3.6 extracted from

  • Bruhmuller, M.; Möhler, H.; Decker, K.
    Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase (1972), Eur. J. Biochem., 29, 143-151.
    View publication on PubMed
Search for more literature for 1.5.3.6

Inhibitors

Inhibitors Comment Organism Structure
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one
-
 Paenarthrobacter nicotinovorans
3-(4-aminobutyl)-pyridine
-
 Paenarthrobacter nicotinovorans
6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans
D,L-2-Hydroxynicotine
-
 Paenarthrobacter nicotinovorans
Phenanthroline inactivation above 1 mM Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 6-hydroxy-D-nicotine
-
 Paenarthrobacter nicotinovorans
0.2
-
 6-amino-D-nicotine
-
 Paenarthrobacter nicotinovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 sedimentation equilibrium and SDS-PAGE Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
additional information Paenarthrobacter nicotinovorans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenarthrobacter nicotinovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.5
-
-
 Paenarthrobacter nicotinovorans

Storage Stability

Storage Stability Organism
4°C, fairly stable at neutral or alkaline pH in presence of 10 mM mercaptoethanol Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-6-aminonicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(-aminopyridin-3-yl)-4-methylamino-butan-1-one + H2O2
-
 ?
(R)-6-hydroxynicotine + H2O + electron acceptor as electron acceptors methylene blue and 2,6-dichlorophenolindophenol aerobically and anaerobically Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + electron donor
-
 ?
(R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?
additional information
-
 Paenarthrobacter nicotinovorans ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Paenarthrobacter nicotinovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 inactivation above this temperature Paenarthrobacter nicotinovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 0.1 M Tris-HCl buffer Paenarthrobacter nicotinovorans
8.5
-
 0.1 M glycine-NaOH buffer Paenarthrobacter nicotinovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 below this pH inactivation Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD one mol FAD per mol enzyme Paenarthrobacter nicotinovorans
FAD covalently bound to a histidine Paenarthrobacter nicotinovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one
-
 Paenarthrobacter nicotinovorans
0.7
-
 3-(4-aminobutyl)-pyridine
-
 Paenarthrobacter nicotinovorans
1.5
-
 6-hydroxy-L-nicotine
-
 Paenarthrobacter nicotinovorans
1.7
-
 D,L-2-Hydroxynicotine
-
 Paenarthrobacter nicotinovorans