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Literature summary for 1.5.3.4 extracted from
- Enzymatic methylation and demethylation of protein-bound lysine residues (1984), Methods Enzymol., 106, 274-287.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | inhibitory toward the partially purified enzyme | Rattus norvegicus |  |
| Co2+ | inhibitory at 1.2 mM concentration | Rattus norvegicus | |
| KCN | 1.3 mM, about 40% inhibition | Rattus norvegicus | |
| Ni2+ | inhibitory at 1.2 mM concentration | Rattus norvegicus | |
| Zn2+ | inhibitory at 1.2 mM concentration | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.05 | - |
N6-methyl-L-lysine | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O | Rattus norvegicus | methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase | demethylated lysine residues + formaldehyde + H2O2 | - |
ir | |
| epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O | Rattus norvegicus | protein: e.g. histones | demethylated lysine residues + formaldehyde + H2O2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 16fold partial purification | Rattus norvegicus |
| enzyme from kidney | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00106 | - |
N6-methyl-L-lysyl-histone | Rattus norvegicus |
| 0.0283 | - |
N6-methyl-L-lysine | Rattus norvegicus |
| 0.0433 | - |
16fold purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-(methylamino)-2-oxohexanoic acid + H2O + O2 | - |
Rattus norvegicus | 6-amino-2-oxohexanoic acid + formaldehyde + H2O2 | - |
? | |
| epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O | methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase | Rattus norvegicus | demethylated lysine residues + formaldehyde + H2O2 | - |
ir | |
| epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O | protein: e.g. histones | Rattus norvegicus | demethylated lysine residues + formaldehyde + H2O2 | - |
ir | |
| additional information | not: delta-N-monomethyl-L-ornithine | Rattus norvegicus | ? | - |
? | |
| N6,N6-dimethyl-L-lysine + O2 + H2O | - |
Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? | |
| N6-methyl-L-lysine + O2 + H2O | - |
Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
ir | |
| N6-methyl-L-lysyl-histone + O2 + H2O | - |
Rattus norvegicus | histone + formaldehyde + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | same enzyme is responsible for the demethylation of epsilon-N-methyl-L-lysine and of methylated histone | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
phosphate buffer | Rattus norvegicus |
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