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Literature summary for 1.5.3.25 extracted from

  • Miura, S.; Ferri, S.; Tsugawa, W.; Kim, S.; Sode, K.
    Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis (2008), Protein Eng. Des. Sel., 21, 233-239 .
    View publication on PubMed
Search for more literature for 1.5.3.25

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling and docking of substrates fructosyl L-valine and Nepsilon-fructosyl-L-lysine. Residue Asn354 interacts closely with Nepsilon-fructosyl-L-lysine, but not with fructosyl L-valine Pichia sp. N1-1

Protein Variants

Protein Variants Comment Organism
N354A mutation leads to an increase in the activity ratio fructosyl L-valine/Nepsilon-fructosyl-L-lysine Pichia sp. N1-1
N354H mutation leads to an increase in the activity ratio fructosyl L-valine/Nepsilon-fructosyl-L-lysine Pichia sp. N1-1
N354K mutation leads to an increase in the activity ratio fructosyl L-valine/Nepsilon-fructosyl-L-lysine Pichia sp. N1-1
N354V mutation leads to an increase in the activity ratio fructosyl L-valine/Nepsilon-fructosyl-L-lysine Pichia sp. N1-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
 fructosyl L-valine mutant N354H, pH 7, 25°C Pichia sp. N1-1
3
-
 fructosyl L-valine mutant N354A, pH 7, 25°C Pichia sp. N1-1
3.9
-
 fructosyl L-valine mutant N354K, pH 7, 25°C Pichia sp. N1-1
5.6
-
 fructosyl L-valine wild-type, pH 7, 25°C Pichia sp. N1-1
9.8
-
 Nepsilon-fructosyl-L-lysine wild-type, pH 7, 25°C Pichia sp. N1-1
10
-
 fructosyl L-valine mutant N354V, pH 7, 25°C Pichia sp. N1-1
63
-
 Nepsilon-fructosyl-L-lysine mutant N354A, pH 7, 25°C Pichia sp. N1-1

Organism

Organism UniProt Comment Textmining
Pichia sp. N1-1 Q3L8U0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl L-valine + O2 + H2O poor substrate of isoform amadoriases I Pichia sp. N1-1 glucosone + L-valine + H2O2
-
 ?
Nepsilon-fructosyl-L-lysine + O2 + H2O poor substrate of isoform amadoriases I Pichia sp. N1-1 glucosone + L-lysine + H2O2
-
 ?