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Literature summary for 1.5.3.25 extracted from
- Recombinant Agrobacterium AgaE-like protein with fructosyl amino acid oxidase activity (2002), Biosci. Biotechnol. Biochem., 66, 2323-2329 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Agrobacterium tumefaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
fructosyl glycine | pH 8.0, 25°C | Agrobacterium tumefaciens | |
| 1.64 | - |
fructosyl L-valine | pH 8.0, 25°C | Agrobacterium tumefaciens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
gel filtration | Agrobacterium tumefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | Q9WWF2 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructosyl glycine + O2 + H2O | - |
Agrobacterium tumefaciens | glucosone + glycine + H2O2 | - |
? |  |
| fructosyl L-valine + O2 + H2O | - |
Agrobacterium tumefaciens | glucosone + L-valine + H2O2 | - |
? | |
| additional information | no substrates: glycated protein or Nepsilon-fructosyl-L-lysine, Nepsilon-fructosyl-Nalpha-(benzyloxycarbonyl)-L-lysine | Agrobacterium tumefaciens | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaE-like protein | - |
Agrobacterium tumefaciens |
| fructosyl amino acid oxidase | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Agrobacterium tumefaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
less than 20% residual activity | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains noncovalently bound FAD as a cofactor | Agrobacterium tumefaciens | |
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Release 2023.1 (January 2023)
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