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Literature summary for 1.5.3.25 extracted from

  • Wu, X.; Chen, S.G.; Petrash, J.M.; Monnier, V.M.
    Alteration of substrate selectivity through mutation of two arginine residues in the binding site of amadoriase II from Aspergillus sp. (2002), Biochemistry, 41, 4453-4458 .
    View publication on PubMed
Search for more literature for 1.5.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus sp.

Protein Variants

Protein Variants Comment Organism
R112E mutation in isoform II to the corresponding residue of isoform I, leads to reversal of the enzymatic activities toward fructosylglycine and fructosylpropylamine Aspergillus sp.
R112E mutation in isoform II, kcat value is similar to the wild-type, while the Km of the mutant toward fructosyl glycine increases significantly Aspergillus sp.
R112E/R114E mutation in isoform II, kcat value is similar to the wild-type, while the Km of the mutant toward fructosyl glycine increases significantly Aspergillus sp.
R114E mutation in isoform II to the corresponding residue of isoform I, leads to reversal of the enzymatic activities toward fructosylglycine and fructosylpropylamine Aspergillus sp.
R114E mutation in isoform II, kcat value is similar to the wild-type, while the Km of the mutant toward fructosyl glycine increases significantly Aspergillus sp.

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylglyoxal inhibition of amadoriase II enzyme activity toward fructosyl glycine, with less effect on the enzymatic activity toward fructosyl propylamine Aspergillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 fructosyl propylamine isoform amadoriase I, pH 8, 37°C Aspergillus sp.
0.23
-
 fructosyl glycine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
0.58
-
 fructosyl propylamine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
0.77
-
 fructosyl glycine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
1.16
-
 fructosyl propylamine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
1.5 2 fructosyl propylamine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
1.59
-
 fructosyl propylamine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
1.93
-
 fructosyl glycine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
2.5 3 fructosyl propylamine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
3.04
-
 fructosyl propylamine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
4.48
-
 fructosyl glycine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
5.11
-
 fructosyl glycine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
8.16
-
 fructosyl glycine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
9.75
-
 fructosyl glycine isoform amadoriase I, pH 8, 37°C Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl glycine + O2 + H2O
-
 Aspergillus sp. glucosone + glycine + H2O2
-
 ?
fructosyl propylamine + O2 + H2O
-
 Aspergillus sp. glucosone + propylamine + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 fructosyl glycine isoform amadoriase I, pH 8, 37°C Aspergillus sp.
2
-
 fructosyl propylamine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
2.1
-
 fructosyl propylamine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
2.7
-
 fructosyl propylamine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
3
-
 fructosyl glycine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
3
-
 fructosyl glycine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
3
-
 fructosyl glycine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
3.1
-
 fructosyl propylamine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
3.2
-
 fructosyl glycine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
3.5
-
 fructosyl glycine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
3.7
-
 fructosyl propylamine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
4.7
-
 fructosyl propylamine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
4.9
-
 fructosyl glycine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
5.8
-
 fructosyl propylamine isoform amadoriase I, pH 8, 37°C Aspergillus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.066
-
 fructosyl glycine isoform amadoriase I, pH 8, 37°C Aspergillus sp.
0.36
-
 fructosyl glycine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
0.7
-
 fructosyl glycine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
1.1
-
 fructosyl propylamine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
1.2
-
 fructosyl propylamine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
1.3
-
 fructosyl propylamine amadoriase II mutant R114E, pH 8, 37°C Aspergillus sp.
1.5
-
 fructosyl glycine amadoriase II mutant R114L, pH 8, 37°C Aspergillus sp.
2.7
-
 fructosyl propylamine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
3.1
-
 fructosyl propylamine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
3.6
-
 fructosyl propylamine amadoriase II mutant R112E/R114E, pH 8, 37°C Aspergillus sp.
3.9
-
 fructosyl glycine amadoriase II mutant R112L, pH 8, 37°C Aspergillus sp.
11
-
 fructosyl glycine amadoriase II mutant R112E, pH 8, 37°C Aspergillus sp.
14
-
 fructosyl glycine amadoriase II wild-type, pH 8, 37°C Aspergillus sp.
250
-
 fructosyl propylamine isoform amadoriase I, pH 8, 37°C Aspergillus sp.