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Literature summary for 1.5.3.25 extracted from

  • Qian, Y.; Zheng, J.; Lin, Z.
    Loop engineering of amadoriase II and mutational cooperativity (2013), Appl. Microbiol. Biotechnol., 97, 8599-8607 .
    View publication on PubMed
Search for more literature for 1.5.3.25

Protein Variants

Protein Variants Comment Organism
N63D/K64Q/R112W/V113F/R114S strong increase in activity with fructosyl adamantanamine and with fructosyl polylysine Aspergillus fumigatus
R112W strong increase in activity with fructosyl adamantanamine Aspergillus fumigatus
R112Y strong increase in activity with fructosyl adamantanamine Aspergillus fumigatus
R112Y/V113F/R114S strong increase in activity with fructosyl adamantanamine Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus P78573
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl adamantanamine + O2 + H2O poor substrate of isoform amadoriases I Aspergillus fumigatus glucosone + adamantanamine + H2O2
-
 ?
fructosyl poly-L-lysine + O2 + H2O
-
 Aspergillus fumigatus glucosone + poly-L-lysine + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 fructosyl adamantanamine mutant R112W, pH 8, temperature not specified in the publication Aspergillus fumigatus
0.008
-
 fructosyl adamantanamine mutant R112Y, pH 8, temperature not specified in the publication Aspergillus fumigatus
0.008
-
 fructosyl poly-L-lysine wild-type, pH 8, temperature not specified in the publication Aspergillus fumigatus
0.016
-
 fructosyl adamantanamine mutant R112Y/V113F/R114S, pH 8, temperature not specified in the publication Aspergillus fumigatus
0.21
-
 fructosyl adamantanamine mutant N63D/K64Q/R112W/V113F/R114S, pH 8, temperature not specified in the publication Aspergillus fumigatus
0.48
-
 fructosyl adamantanamine wild-type, pH 8, temperature not specified in the publication Aspergillus fumigatus
2.55
-
 fructosyl adamantanamine mutant R112Y, pH 8, temperature not specified in the publication Aspergillus fumigatus
2.9
-
 fructosyl adamantanamine mutant N63D/K64Q/R112W/V113F/R114S, pH 8, temperature not specified in the publication Aspergillus fumigatus
5.5
-
 fructosyl adamantanamine mutant R112W, pH 8, temperature not specified in the publication Aspergillus fumigatus
11.9
-
 fructosyl adamantanamine mutant R112Y/V113F/R114S, pH 8, temperature not specified in the publication Aspergillus fumigatus