Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.25 extracted from

  • Mennella, C.; Borrelli, R.C.; Vinale, F.; Ruocco, M.; Fogliano, V.
    Substrate specificity of amadoriase I from Aspergillus fumigatus (2005), Ann. N. Y. Acad. Sci., 1043, 837-844 .
    View publication on PubMed
Search for more literature for 1.5.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified by Ni His-tag affinity chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructosyl L-lysine + O2 + H2O
-
 Aspergillus fumigatus glucosone + L-lysine + H2O2
-
 ?
additional information Kmvalue decreases with the length of peptide substrate, being slightly reduced for dipeptides, and is around 10 mM for tri- and tetrapeptides. Glycated proteins are not substrates of the enzyme, but when amadoriase I is added during the glycation reaction, a significant reduction of Amadori product formation is observed on both peptides and proteins Aspergillus fumigatus ?
-
-
Nepsilon-fructosyl L-Lys-L-Leu + O2 + H2O
-
 Aspergillus fumigatus glucosone + L-Lys-L-Leu + H2O2
-
 ?
Nepsilon-fructosyl L-Lys-L-Phe + O2 + H2O
-
 Aspergillus fumigatus glucosone + L-Lys-L-Phe + H2O2
-
 ?
Nepsilon-fructosyl Nalpha-acetyl-L-Lys-D-Ala-L-Ala + O2 + H2O
-
 Aspergillus fumigatus glucosone + Nalpha-acetyl-L-Lys-D-Ala-L-Ala + H2O2
-
 ?
Nepsilon-fructosyl Nalpha-acetyl-L-Lys-D-D-Lys-Sar-Glu + O2 + H2O
-
 Aspergillus fumigatus glucosone + Nalpha-acetyl-L-Lys-D-Lys-Sar-Glu + H2O2
-
 ?
Nepsilon-fructosyl Nalpha-acetyl-L-lysine-N-methylamide + O2 + H2O
-
 Aspergillus fumigatus glucosone + acetyl-L-lysine-N-methylamide + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, isoform amadoriase I, SDS-PAGE Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.54
-
 Nepsilon-fructosyl Nalpha-acetyl-L-lysine-N-methylamide pH 7.4, 37°C Aspergillus fumigatus
4.2
-
 fructosyl L-lysine pH 7.4, 37°C Aspergillus fumigatus
5.6
-
 Nepsilon-fructosyl L-Lys-L-Phe pH 7.4, 37°C Aspergillus fumigatus
6.36
-
 Nepsilon-fructosyl L-Lys-L-Leu pH 7.4, 37°C Aspergillus fumigatus
9.6
-
 Nepsilon-fructosyl Nalpha-acetyl-L-Lys-D-Ala-L-Ala pH 7.4, 37°C Aspergillus fumigatus
10.8
-
 fructosyl L-lysine pH 7.4, 37°C Aspergillus fumigatus