Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Corynebacterium sp. U-96 |
Protein Variants | Comment | Organism |
---|---|---|
K171A | the mutant shows 39% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K171D | the mutant shows 32% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K171R | the mutant shows 58% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K358A | inactive | Corynebacterium sp. U-96 |
K358D | inactive | Corynebacterium sp. U-96 |
K358R | the mutant shows 0.07% activity and higher apparent Km for sarcosine than the wild type enzyme | Corynebacterium sp. U-96 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0312 | - |
O2 | mutant enzyme K171R, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
0.0329 | - |
O2 | wild type enzyme, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
0.386 | - |
O2 | mutant enzyme K171A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
0.73 | - |
O2 | mutant enzyme K171D, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
1.47 | - |
sarcosine | mutant enzyme K171A, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
1.72 | - |
sarcosine | mutant enzyme K171R, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
2.75 | - |
sarcosine | mutant enzyme K171D, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
2.76 | - |
sarcosine | wild type enzyme, at pH 8.0 and 25°C | Corynebacterium sp. U-96 | |
17.4 | - |
sarcosine | mutant enzyme K358R, at pH 8.0 and 25°C | Corynebacterium sp. U-96 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. U-96 | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. U-96 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
heterotetrameric sarcosine oxidase | - |
Corynebacterium sp. U-96 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Corynebacterium sp. U-96 | |
FMN | - |
Corynebacterium sp. U-96 |