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Literature summary for 1.5.3.24 extracted from
- Kinetic studies of the mechanism of carbon-hydrogen bond breakage by the heterotetrameric sarcosine oxidase of Arthrobacter sp. 1-IN (2000), Biochemistry, 39, 1189-1198 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Arthrobacter sp. 1-IN |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Arthrobacter sp. 1-IN | - |
glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. 1-IN | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DE-52 column chromatography, phenyl Sepharose column chromatography, and Q-Sepharose column chromatography | Arthrobacter sp. 1-IN |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Arthrobacter sp. 1-IN | glycine + 5,10-methylenetetrahydrofolate + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heterotetrameric sarcosine oxidase | - |
Arthrobacter sp. 1-IN |
| TSOX | - |
Arthrobacter sp. 1-IN |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arthrobacter sp. 1-IN | |
| FMN | - |
Arthrobacter sp. 1-IN | |
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