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Literature summary for 1.5.3.23 extracted from

  • Sviridov, A.V.; Zelenkova, N.F.; Vinokurova, N.G.; Ermakova, I.T.; Leontievsky, A.A.
    New approaches to identification and activity estimation of glyphosate degradation enzymes (2011), Biochemistry (Moscow), 76, 720-725.
    View publication on PubMed
Search for more literature for 1.5.3.23

Application

Application Comment Organism
analysis spectrophotometric method for determining glyphospate oxidoreductase activity in cell-free extract based on the rate of glyoxylate hydrazone formation Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi
-
-
-
Brucella anthropi GPK 3
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
 spectrophotometric determination of product, pH 8.0, 30°C Brucella anthropi
0.034
-
 determination of product by HPLC, pH 8.0, 30°C Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 glyphosate + O2
-
 Brucella anthropi 2 aminomethylphosphonate + 2 glyoxylate
-
 ?
2 glyphosate + O2
-
 Brucella anthropi GPK 3 2 aminomethylphosphonate + 2 glyoxylate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Brucella anthropi

General Information

General Information Comment Organism
physiological function glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains. In Ochrobactrum anthropi GPK3 the initial cleavage reaction is catalyzed by glyphosate oxidoreductase with the formation of aminomethylphosphonic acid and glyoxylate, whereas Achromobacter sp. MPS12 utilize C-P lyase, forming sarcosine Brucella anthropi