Data extracted from this reference:
Cloned(Commentary)
Methanobrevibacter arboriphilus
Methanothermobacter marburgensis
Inhibitors
additional information
the reaction is not inhibited by cyanide
Methanobrevibacter arboriphilus
additional information
the reaction is not inhibited by cyanide
Methanothermobacter marburgensis
KM Value [mM]
0.002
O2
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.002
O2
pH 7.6, 37°C
Methanothermobacter marburgensis
0.03
reduced coenzyme F420
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.03
reduced coenzyme F420
pH 7.6, 37°C
Methanothermobacter marburgensis
Metals/Ions
Fe2+
the 45000 Da protein harbors a binuclear iron center
Methanobrevibacter arboriphilus
Iron
the 45000 Da protein harbors a binuclear iron center
Methanothermobacter marburgensis
Molecular Weight [Da]
43000
x * 43000, SDS-PAGE
Methanothermobacter marburgensis
45000
x * 45000, SDS-PAGE
Methanobrevibacter arboriphilus
45300
x * 45300, calculated from sequence
Methanothermobacter marburgensis
46100
x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
Natural Substrates/ Products (Substrates)
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis DSM 2133
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus DSM Z 744
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
Organism
Methanobrevibacter arboriphilus
B1A7S3
Methanobrevibacter arboriphilus DSM Z 744
B1A7S3
Methanothermobacter marburgensis
Q50497
Methanothermobacter marburgensis DSM 2133
Q50497
Purification (Commentary)
Methanothermobacter marburgensis
Methanobrevibacter arboriphilus
Specific Activity [micromol/min/mg]
0.4
pH 7.6, 37°C, cell suspension
Methanobrevibacter arboriphilus
55
pH 7.6, 37°C, purified enzyme
Methanobrevibacter arboriphilus
Substrates and Products (Substrate)
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
?
Subunits
?
x * 45000, SDS-PAGE; x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
?
x * 43000, SDS-PAGE; x * 45300, calculated from sequence
Methanothermobacter marburgensis
Synonyms
FprA
Methanobrevibacter arboriphilus
FprA
Methanothermobacter marburgensis
Temperature Optimum [°C]
37
assay at
Methanobrevibacter arboriphilus
65
assay at
Methanothermobacter marburgensis
pH Optimum
7.5
Methanobrevibacter arboriphilus
7.5
Methanothermobacter marburgensis
pH Range
6.5
8.5
30% of the maximal activity at pH 6.5 and 8.5
Methanobrevibacter arboriphilus
Cofactor
FMN
the 45000 Da protein contains 1 FMN per mol
Methanobrevibacter arboriphilus
FMN
the 45000 Da protein contains 1 FMN per mol
Methanothermobacter marburgensis
pI Value
Methanobrevibacter arboriphilus
calculated from sequence
5.3
Cloned(Commentary) (protein specific)
Methanobrevibacter arboriphilus
Methanothermobacter marburgensis
Cofactor (protein specific)
FMN
the 45000 Da protein contains 1 FMN per mol
Methanobrevibacter arboriphilus
FMN
the 45000 Da protein contains 1 FMN per mol
Methanothermobacter marburgensis
Inhibitors (protein specific)
additional information
the reaction is not inhibited by cyanide
Methanobrevibacter arboriphilus
additional information
the reaction is not inhibited by cyanide
Methanothermobacter marburgensis
KM Value [mM] (protein specific)
0.002
O2
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.002
O2
pH 7.6, 37°C
Methanothermobacter marburgensis
0.03
reduced coenzyme F420
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.03
reduced coenzyme F420
pH 7.6, 37°C
Methanothermobacter marburgensis
Metals/Ions (protein specific)
Fe2+
the 45000 Da protein harbors a binuclear iron center
Methanobrevibacter arboriphilus
Iron
the 45000 Da protein harbors a binuclear iron center
Methanothermobacter marburgensis
Molecular Weight [Da] (protein specific)
43000
x * 43000, SDS-PAGE
Methanothermobacter marburgensis
45000
x * 45000, SDS-PAGE
Methanobrevibacter arboriphilus
45300
x * 45300, calculated from sequence
Methanothermobacter marburgensis
46100
x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
Natural Substrates/ Products (Substrates) (protein specific)
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis DSM 2133
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus DSM Z 744
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
?
Purification (Commentary) (protein specific)
Methanothermobacter marburgensis
Methanobrevibacter arboriphilus
Specific Activity [micromol/min/mg] (protein specific)
0.4
pH 7.6, 37°C, cell suspension
Methanobrevibacter arboriphilus
55
pH 7.6, 37°C, purified enzyme
Methanobrevibacter arboriphilus
Substrates and Products (Substrate) (protein specific)
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
?
Subunits (protein specific)
?
x * 45000, SDS-PAGE; x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
?
x * 43000, SDS-PAGE; x * 45300, calculated from sequence
Methanothermobacter marburgensis
Temperature Optimum [°C] (protein specific)
37
assay at
Methanobrevibacter arboriphilus
65
assay at
Methanothermobacter marburgensis
pH Optimum (protein specific)
7.5
Methanobrevibacter arboriphilus
7.5
Methanothermobacter marburgensis
pH Range (protein specific)
6.5
8.5
30% of the maximal activity at pH 6.5 and 8.5
Methanobrevibacter arboriphilus
pI Value (protein specific)
Methanobrevibacter arboriphilus
calculated from sequence
5.3
General Information
physiological function
the enzyme is involved in O2 detoxification
Methanobrevibacter arboriphilus
physiological function
the enzyme is involved in O2 detoxification
Methanothermobacter marburgensis
General Information (protein specific)
physiological function
the enzyme is involved in O2 detoxification
Methanobrevibacter arboriphilus
physiological function
the enzyme is involved in O2 detoxification
Methanothermobacter marburgensis
Other publictions for EC 1.5.3.22
724956
Seedorf
Structure of coenzyme F420H2 o ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
FEBS J.
274
1588-1599
2007
-
-
1
1
-
-
-
-
-
1
-
2
-
6
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724955
Seedorf
Si-face stereospecificity at C ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
FEBS J.
272
5337-5342
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724138
Seedorf
F420H2 oxidase (FprA) from Met ...
Methanobrevibacter arboriphilus, Methanobrevibacter arboriphilus DSM Z 744, Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
Arch. Microbiol.
182
126-137
2004
-
-
2
-
-
-
2
4
-
2
4
4
-
6
-
-
2
-
-
-
2
-
8
2
2
2
-
-
-
2
1
-
2
-
1
-
-
-
2
2
-
-
-
-
2
-
4
-
2
4
4
-
-
-
2
-
-
2
-
8
2
2
-
-
-
2
1
-
1
-
2
2
-
-
-