BRENDA - Enzyme Database show
show all sequences of 1.5.3.22

F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification

Seedorf, H.; Dreisbach, A.; Hedderich, R.; Shima, S.; Thauer, R.K.; Arch. Microbiol. 182, 126-137 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Methanobrevibacter arboriphilus
-
Methanothermobacter marburgensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the reaction is not inhibited by cyanide
Methanobrevibacter arboriphilus
additional information
the reaction is not inhibited by cyanide
Methanothermobacter marburgensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
O2
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.002
-
O2
pH 7.6, 37°C
Methanothermobacter marburgensis
0.03
-
reduced coenzyme F420
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.03
-
reduced coenzyme F420
pH 7.6, 37°C
Methanothermobacter marburgensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
the 45000 Da protein harbors a binuclear iron center
Methanobrevibacter arboriphilus
Iron
the 45000 Da protein harbors a binuclear iron center
Methanothermobacter marburgensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
x * 43000, SDS-PAGE
Methanothermobacter marburgensis
45000
-
x * 45000, SDS-PAGE
Methanobrevibacter arboriphilus
45300
-
x * 45300, calculated from sequence
Methanothermobacter marburgensis
46100
-
x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis DSM 2133
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus DSM Z 744
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanobrevibacter arboriphilus
B1A7S3
-
-
Methanobrevibacter arboriphilus DSM Z 744
B1A7S3
-
-
Methanothermobacter marburgensis
Q50497
-
-
Methanothermobacter marburgensis DSM 2133
Q50497
-
-
Purification (Commentary)
Commentary
Organism
-
Methanobrevibacter arboriphilus
-
Methanothermobacter marburgensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.4
-
pH 7.6, 37°C, cell suspension
Methanobrevibacter arboriphilus
55
-
pH 7.6, 37°C, purified enzyme
Methanobrevibacter arboriphilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE; x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
?
x * 43000, SDS-PAGE; x * 45300, calculated from sequence
Methanothermobacter marburgensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanobrevibacter arboriphilus
65
-
assay at
Methanothermobacter marburgensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Methanobrevibacter arboriphilus
7.5
-
-
Methanothermobacter marburgensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
30% of the maximal activity at pH 6.5 and 8.5
Methanobrevibacter arboriphilus
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
the 45000 Da protein contains 1 FMN per mol
Methanobrevibacter arboriphilus
FMN
the 45000 Da protein contains 1 FMN per mol
Methanothermobacter marburgensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Methanobrevibacter arboriphilus
calculated from sequence
-
5.3
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Methanobrevibacter arboriphilus
-
Methanothermobacter marburgensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
the 45000 Da protein contains 1 FMN per mol
Methanobrevibacter arboriphilus
FMN
the 45000 Da protein contains 1 FMN per mol
Methanothermobacter marburgensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the reaction is not inhibited by cyanide
Methanobrevibacter arboriphilus
additional information
the reaction is not inhibited by cyanide
Methanothermobacter marburgensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
O2
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.002
-
O2
pH 7.6, 37°C
Methanothermobacter marburgensis
0.03
-
reduced coenzyme F420
pH 7.6, 37°C
Methanobrevibacter arboriphilus
0.03
-
reduced coenzyme F420
pH 7.6, 37°C
Methanothermobacter marburgensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
the 45000 Da protein harbors a binuclear iron center
Methanobrevibacter arboriphilus
Iron
the 45000 Da protein harbors a binuclear iron center
Methanothermobacter marburgensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
x * 43000, SDS-PAGE
Methanothermobacter marburgensis
45000
-
x * 45000, SDS-PAGE
Methanobrevibacter arboriphilus
45300
-
x * 45300, calculated from sequence
Methanothermobacter marburgensis
46100
-
x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanothermobacter marburgensis DSM 2133
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
2 reduced coenzyme F420 + O2
Methanobrevibacter arboriphilus DSM Z 744
the enzyme is involved in O2 detoxification
2 oxidized coenzyme F420 + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanobrevibacter arboriphilus
-
Methanothermobacter marburgensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.4
-
pH 7.6, 37°C, cell suspension
Methanobrevibacter arboriphilus
55
-
pH 7.6, 37°C, purified enzyme
Methanobrevibacter arboriphilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanothermobacter marburgensis DSM 2133
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme is involved in O2 detoxification
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
2 reduced coenzyme F420 + O2
the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor
724138
Methanobrevibacter arboriphilus DSM Z 744
2 oxidized coenzyme F420 + 2 H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE; x * 46100, calculated from sequence
Methanobrevibacter arboriphilus
?
x * 43000, SDS-PAGE; x * 45300, calculated from sequence
Methanothermobacter marburgensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Methanobrevibacter arboriphilus
65
-
assay at
Methanothermobacter marburgensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Methanobrevibacter arboriphilus
7.5
-
-
Methanothermobacter marburgensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.5
30% of the maximal activity at pH 6.5 and 8.5
Methanobrevibacter arboriphilus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Methanobrevibacter arboriphilus
calculated from sequence
-
5.3
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in O2 detoxification
Methanobrevibacter arboriphilus
physiological function
the enzyme is involved in O2 detoxification
Methanothermobacter marburgensis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in O2 detoxification
Methanobrevibacter arboriphilus
physiological function
the enzyme is involved in O2 detoxification
Methanothermobacter marburgensis
Other publictions for EC 1.5.3.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724956
Seedorf
Structure of coenzyme F420H2 o ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
FEBS J.
274
1588-1599
2007
-
-
1
1
-
-
-
-
-
1
-
2
-
6
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724955
Seedorf
Si-face stereospecificity at C ...
Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
FEBS J.
272
5337-5342
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
724138
Seedorf
F420H2 oxidase (FprA) from Met ...
Methanobrevibacter arboriphilus, Methanobrevibacter arboriphilus DSM Z 744, Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133
Arch. Microbiol.
182
126-137
2004
-
-
2
-
-
-
2
4
-
2
4
4
-
6
-
-
2
-
-
-
2
-
8
2
2
-
-
-
2
1
-
2
-
1
-
-
-
2
2
-
-
-
-
2
-
4
-
2
4
4
-
-
-
2
-
-
2
-
8
2
2
-
-
-
2
1
-
1
-
2
2
-
-
-