F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification

Seedorf, H.; Dreisbach, A.; Hedderich, R.; Shima, S.; Thauer, R.K.; Arch. Microbiol. 182, 126-137 (2004)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.002

-

O2

pH 7.6, 37°C

Methanobrevibacter arboriphilus

0.002

-

O2

pH 7.6, 37°C

Methanothermobacter marburgensis

0.03

-

reduced coenzyme F420

pH 7.6, 37°C

Methanobrevibacter arboriphilus

0.03

-

reduced coenzyme F420

pH 7.6, 37°C

Methanothermobacter marburgensis

Metals/Ions

Commentary

Organism

Structure

Fe2+

the 45000 Da protein harbors a binuclear iron center

Methanobrevibacter arboriphilus

Iron

the 45000 Da protein harbors a binuclear iron center

Methanothermobacter marburgensis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2 reduced coenzyme F420 + O2

Methanothermobacter marburgensis

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanobrevibacter arboriphilus

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanothermobacter marburgensis DSM 2133

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanobrevibacter arboriphilus DSM Z 744

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanothermobacter marburgensis

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanobrevibacter arboriphilus

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanothermobacter marburgensis

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanobrevibacter arboriphilus

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanothermobacter marburgensis DSM 2133

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanothermobacter marburgensis DSM 2133

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanobrevibacter arboriphilus DSM Z 744

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanobrevibacter arboriphilus DSM Z 744

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

Cofactor

Commentary

Organism

Structure

FMN

the 45000 Da protein contains 1 FMN per mol

Methanobrevibacter arboriphilus

FMN

the 45000 Da protein contains 1 FMN per mol

Methanothermobacter marburgensis

Cofactor

Commentary

Organism

Structure

FMN

the 45000 Da protein contains 1 FMN per mol

Methanobrevibacter arboriphilus

FMN

the 45000 Da protein contains 1 FMN per mol

Methanothermobacter marburgensis

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.002

-

O2

pH 7.6, 37°C

Methanobrevibacter arboriphilus

0.002

-

O2

pH 7.6, 37°C

Methanothermobacter marburgensis

0.03

-

reduced coenzyme F420

pH 7.6, 37°C

Methanobrevibacter arboriphilus

0.03

-

reduced coenzyme F420

pH 7.6, 37°C

Methanothermobacter marburgensis

Metals/Ions

Commentary

Organism

Structure

Fe2+

the 45000 Da protein harbors a binuclear iron center

Methanobrevibacter arboriphilus

Iron

the 45000 Da protein harbors a binuclear iron center

Methanothermobacter marburgensis

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

2 reduced coenzyme F420 + O2

Methanothermobacter marburgensis

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanobrevibacter arboriphilus

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanothermobacter marburgensis DSM 2133

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

2 reduced coenzyme F420 + O2

Methanobrevibacter arboriphilus DSM Z 744

the enzyme is involved in O2 detoxification

2 oxidized coenzyme F420 + 2 H2O

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanothermobacter marburgensis

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanobrevibacter arboriphilus

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanothermobacter marburgensis

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanobrevibacter arboriphilus

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanothermobacter marburgensis DSM 2133

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanothermobacter marburgensis DSM 2133

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme is involved in O2 detoxification

724138

Methanobrevibacter arboriphilus DSM Z 744

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

2 reduced coenzyme F420 + O2

the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor

724138

Methanobrevibacter arboriphilus DSM Z 744

2 oxidized coenzyme F420 + 2 H2O

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

724956

Seedorf

Structure of coenzyme F420H2 o ...

Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133

FEBS J.

274

1588-1599

2007

-

-

1

1

-

-

-

-

-

1

-

2

-

6

-

-

1

-

-

-

-

-

4

-

1

-

-

-

-

-

-

-

1

-

-

-

-

-

1

1

1

-

-

-

-

-

-

-

1

-

2

-

-

-

1

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

724955

Seedorf

Si-face stereospecificity at C ...

Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133

FEBS J.

272

5337-5342

2005

-

-

-

-

-

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

2

-

1

1

-

-

-

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

-

1

-

-

-

1

-

-

-

-

-

-

-

-

-

724138

Seedorf

F420H2 oxidase (FprA) from Met ...

Methanobrevibacter arboriphilus, Methanobrevibacter arboriphilus DSM Z 744, Methanothermobacter marburgensis, Methanothermobacter marburgensis DSM 2133

Arch. Microbiol.

182

126-137

2004

-

-

2

-

-

-

2

4

-

2

4

4

-

6

-

-

2

-

-

-

2

-

8

2

2

2

-

-

-

2

1

-

2

-

1

-

-

-

2

2

-

-

-

-

2

-

4

-

2

4

4

-

-

-

2

-

-

2

-

8

2

2

-

-

-

2

1

-

1

-

2

2

-

-

-