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Literature summary for 1.5.3.19 extracted from
- A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 (2004), Eur. J. Biochem., 271, 4677-4684.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Paenarthrobacter nicotinovorans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H67A | mutation results in resulted in a protein without covalently bound flavin. Mutant protein contains noncovalently bound flavin and exhibits 10% of the enzyme activity of the wild-type | Paenarthrobacter nicotinovorans |
| W66S | mutation results in resulted in a protein without covalently bound flavin and complete loss of actiovity | Paenarthrobacter nicotinovorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
4-methylaminobutanoate | pH 10, temperature not specified in the publication | Paenarthrobacter nicotinovorans |  |
| 25 | - |
sarcosine | pH 10, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 89395 | - |
1 * 90000, SDS-PAGE, 1 * 89395, calculated | Paenarthrobacter nicotinovorans |
| 90000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenarthrobacter nicotinovorans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylaminobutanoate + O2 + H2O | - |
Paenarthrobacter nicotinovorans | 4-aminobutanoate + formaldehyde + H2O2 | - |
? | |
| additional information | no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid | Paenarthrobacter nicotinovorans | ? | - |
? | |
| sarcosine + O2 + H2O | about 200fold decrease in rate compared to 4-methylaminobutanoate | Paenarthrobacter nicotinovorans | glycine + formaldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE, 1 * 89395, calculated | Paenarthrobacter nicotinovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-N-methylaminobutanoate oxidase | - |
Paenarthrobacter nicotinovorans |
| gamma-N-methylaminobutyrate oxidase | - |
Paenarthrobacter nicotinovorans |
| mabO | - |
Paenarthrobacter nicotinovorans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
sarcosine | pH 10, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
| 800 | - |
4-methylaminobutanoate | pH 10, temperature not specified in the publication | Paenarthrobacter nicotinovorans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Paenarthrobacter nicotinovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | 1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor | Paenarthrobacter nicotinovorans | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Paenarthrobacter nicotinovorans | expression only upon growth in presence of nicotine | up |
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Release 2023.1 (January 2023)
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