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Literature summary for 1.5.3.17 extracted from

  • Wang, H.; Liu, B.; Li, H.; Zhang, S.
    Identification and biochemical characterization of polyamine oxidases in amphioxus Implications for emergence of vertebrate-specific spermine and acetylpolyamine oxidases (2016), Gene, 575, 429-437 .
    View publication on PubMed
Search for more literature for 1.5.3.17

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes expressed in Escherichia coli Branchiostoma japonicum

Protein Variants

Protein Variants Comment Organism
H64E Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
H64Q Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
H69E compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
H69Q compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
K301M Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
K315M compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
T460S Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
T460Y Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym Branchiostoma japonicum
T467S compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum
T467Y compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine Branchiostoma japonicum

Inhibitors

Inhibitors Comment Organism Structure
N1,N4-bis(2,3-butadienyl)-1,4-butanediamine 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of N1-acetylspermine oxidation; 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of spermine oxidation Branchiostoma japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 spermidine 37°C, pH 9.5, wild-type enzyme Branchiostoma japonicum
0.00836
-
 spermine 37°C, pH 8.5, wild-type enzyme Branchiostoma japonicum
0.012
-
 spermine 37°C, pH 8.5, mutant enzyme T460S Branchiostoma japonicum
0.0124
-
 spermidine 37°C, pH 9.5, wild-type enzyme Branchiostoma japonicum
0.013
-
 spermine 37°C, pH 8.5, mutant enzyme T460Y Branchiostoma japonicum
0.016
-
 spermidine 37°C, pH 9.5, mutant enzyme K301M Branchiostoma japonicum
0.017
-
 spermine 37°C, pH 8.5, mutant enzyme K301M Branchiostoma japonicum
0.017
-
 spermidine 37°C, pH 9.5, mutant enzyme T460Y Branchiostoma japonicum
0.019
-
 spermidine 37°C, pH 9.5, mutant enzyme H64E Branchiostoma japonicum
0.019
-
 spermidine 37°C, pH 9.5, mutant enzyme T460S Branchiostoma japonicum
0.02
-
 spermidine 37°C, pH 9.5, mutant enzyme T467Y Branchiostoma japonicum
0.021
-
 spermidine 37°C, pH 9.5, mutant enzyme H64Q Branchiostoma japonicum
0.0229
-
 spermidine 37°C, pH 9.5, mutant enzyme H69Q Branchiostoma japonicum
0.024
-
 spermine 37°C, pH 8.5, mutant enzyme H64E Branchiostoma japonicum
0.025
-
 spermidine 37°C, pH 9.5, mutant enzyme H69E Branchiostoma japonicum
0.0269
-
 spermine 37°C, pH 8.5, mutant enzyme H64Q Branchiostoma japonicum
0.031
-
 spermidine 37°C, pH 9.5, mutant enzyme K315M Branchiostoma japonicum
0.0369
-
 N1-acetylspermine 37°C, pH 8.0, wild-type enzyme Branchiostoma japonicum
0.043
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme K315M Branchiostoma japonicum
0.046
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467Y Branchiostoma japonicum
0.058
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme T467S Branchiostoma japonicum
0.062
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69E Branchiostoma japonicum
0.071
-
 N1-acetylspermine 37°C, pH 8.0, mutant enzyme H69Q Branchiostoma japonicum
0.182
-
 spermidine 37°C, pH 9.5, mutant enzyme T467S Branchiostoma japonicum

Organism

Organism UniProt Comment Textmining
Branchiostoma japonicum
-
-
-
Branchiostoma japonicum A0A0N7IZL9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Branchiostoma japonicum

Source Tissue

Source Tissue Comment Organism Textmining
gill
-
 Branchiostoma japonicum
-
hepatic cecum
-
 Branchiostoma japonicum
-
hindgut
-
 Branchiostoma japonicum
-
muscle
-
 Branchiostoma japonicum
-
notochord
-
 Branchiostoma japonicum
-
notochord Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-
ovary
-
 Branchiostoma japonicum
-
ovary Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-
testis
-
 Branchiostoma japonicum
-
testis Bjpao1 is predominantly expressed in the notochord, testis and ovary Branchiostoma japonicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Bjpao1 shows no activity with N1-acetylspermine Branchiostoma japonicum ?
-
 ?
additional information Bjpao2 shows no activity with spermine Branchiostoma japonicum ?
-
 ?
N1-acetylspermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-acetamidopropanal + H2O2
-
 ?
spermidine + O2 + H2O
-
 Branchiostoma japonicum putrescine + 3-aminopropanal + H2O2
-
 ?
spermine + O2 + H2O
-
 Branchiostoma japonicum spermidine + 3-aminopropanal + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Branchiostoma japonicum
? x * 59000, SDS-PAGE Branchiostoma japonicum

Synonyms

Synonyms Comment Organism
Bjpao1
-
 Branchiostoma japonicum
Bjpao2
-
 Branchiostoma japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Branchiostoma japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 substrate: N1-acetylspermine Branchiostoma japonicum
8.5
-
 substrate: spermine Branchiostoma japonicum
9
-
 substrate: spermidine Branchiostoma japonicum

pI Value

Organism Comment pI Value Maximum pI Value
Branchiostoma japonicum calculated from sequence
-
 4.9
Branchiostoma japonicum calculated from sequence
-
 5.13