Cloned (Comment) | Organism |
---|---|
AtPAO2, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method | Arabidopsis thaliana |
AtPAO3, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, recombinant expression as maltose-binding protein fusion protein in Escherichia coli, and expression of GFP-tagged AtPAO3 in transgenic Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method resulting in increased putrescine levels after feeding of exogenous spermidine | Arabidopsis thaliana |
AtPAO4, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | homozygous lines of T-DNA insertional mutant of Arabidopsis thaliana SALK_121288 for the AtPAO3 gene | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guazatine | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
0.045 | - |
norspermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.047 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.05 | - |
thermospermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.139 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.233 | - |
N1-acetylspermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
0.27 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
0.274 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.409 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
0.58 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
2 | - |
N1-acetylspermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | AtPAO4 is rapidly inactivated under high ionic strength conditions | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-acetylspermidine + O2 + H2O | Arabidopsis thaliana | - |
putrescine + 3-acetamidopropanal + H2O2 | - |
? | |
N1-acetylspermidine + O2 + H2O | Arabidopsis thaliana Columbia-0 | - |
putrescine + 3-acetamidopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | Arabidopsis thaliana | - |
spermidine + N-acetyl-3-aminopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | Arabidopsis thaliana Columbia-0 | - |
spermidine + N-acetyl-3-aminopropanal + H2O2 | - |
? | |
spermidine + O2 + H2O | Arabidopsis thaliana | - |
putrescine + 3-aminobutanal + H2O2 | - |
? | |
spermidine + O2 + H2O | Arabidopsis thaliana Columbia-0 | - |
putrescine + 3-aminobutanal + H2O2 | - |
? | |
spermine + O2 + H2O | Arabidopsis thaliana | - |
spermidine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | Arabidopsis thaliana Columbia-0 | - |
spermidine + 3-aminopropanal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9LYT1 | gene AtPAO3 | - |
Arabidopsis thaliana | Q9SKX5 | - |
- |
Arabidopsis thaliana Columbia-0 | - |
- |
- |
Arabidopsis thaliana Columbia-0 | Q9LYT1 | gene AtPAO3 | - |
Arabidopsis thaliana Columbia-0 | Q9SKX5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
recombinant maltose-binding protein fusion enzyme from Escherichia coli by amylose affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-acetylspermidine + O2 + H2O | - |
Arabidopsis thaliana | putrescine + 3-acetamidopropanal + H2O2 | - |
? | |
N1-acetylspermidine + O2 + H2O | - |
Arabidopsis thaliana Columbia-0 | putrescine + 3-acetamidopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + N-acetyl-3-aminopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Arabidopsis thaliana Columbia-0 | spermidine + N-acetyl-3-aminopropanal + H2O2 | - |
? | |
norspermine + O2 + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
norspermine + O2 + H2O | - |
Arabidopsis thaliana Columbia-0 | ? | - |
? | |
spermidine + O2 + H2O | - |
Arabidopsis thaliana | putrescine + 3-aminobutanal + H2O2 | - |
? | |
spermidine + O2 + H2O | - |
Arabidopsis thaliana Columbia-0 | putrescine + 3-aminobutanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Arabidopsis thaliana Columbia-0 | spermidine + 3-aminopropanal + H2O2 | - |
? | |
thermospermine + O2 + H2O | - |
Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPAO2 | - |
Arabidopsis thaliana |
AtPAO3 | - |
Arabidopsis thaliana |
AtPAO4 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
N1-acetylspermine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.02 | - |
N1-acetylspermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.1 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.1 | - |
thermospermine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.4 | - |
thermospermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
0.45 | - |
norspermine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana | |
0.5 | - |
thermospermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.8 | - |
N1-acetylspermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
1.1 | - |
norspermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
1.7 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
2.9 | - |
norspermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
3.4 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO3 | Arabidopsis thaliana | |
4.2 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
4.6 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO2 | Arabidopsis thaliana | |
4.6 | - |
spermine | pH 7.5, 25°C, recombinant AtPAO4 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | dependent on the substrate | Arabidopsis thaliana |
7.5 | - |
- |
Arabidopsis thaliana |
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | AtPAO2-AtPAO4 form a subfamily of polyamine oxxidases different from AtPAO1, EC 1.5.3.16, overview | Arabidopsis thaliana |
metabolism | the enzyme is involved in the polyamine back-conversion pathway, overview | Arabidopsis thaliana |
physiological function | plant PAOs oxidize the carbon at the endo-side of the N4-nitrogen of Spd and Spm, producing 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal, respectively, in addition to 1,3-diaminopropane and H2O2, and are considered to be involved in terminal catabolism of polyamines, physiological role(s) of the polyamine catabolic pathways in plants, overview. All AtPAOs characterized are involved in a polyamine backconversion pathway | Arabidopsis thaliana |