Cloned (Comment) | Organism |
---|---|
AtPAO1, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guazatine | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Arabidopsis thaliana | |
0.12 | - |
spermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
0.47 | - |
N1-acetylspermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
5.7 | - |
thermospermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
6.9 | - |
norspermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermine + O2 + H2O | Arabidopsis thaliana | - |
spermidine + 3-aminopropanal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana Columbia-0 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no oxidation activity with spermidine | Arabidopsis thaliana | ? | - |
? | |
N1-acetylspermidine + O2 + H2O | - |
Arabidopsis thaliana | putrescine + 3-acetamidopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-acetamidopropanal + H2O2 | - |
? | |
norspermine + O2 + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
spermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? | |
thermospermine + O2 + H2O | - |
Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPAO1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
thermospermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
0.09 | - |
norspermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
0.2 | - |
N1-acetylspermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana | |
2.5 | - |
spermine | pH 8.0, 25°C, recombinant AtPAO1 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | AtPAO2-AtPAO4 form a subfamily of polyamine oxidases different from AtPAO1, overview | Arabidopsis thaliana |