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Literature summary for 1.5.3.16 extracted from
- Heterologous expression and characterization of mouse spermine oxidase (2003), J. Biol. Chem., 278, 5271-5276.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| subcloned and expressed in secreted and secreted-tagged forms into Escherichia coli BL21 DE3 cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| MDL72527 | - |
Mus musculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
spermine | pH 8.0, 37°C, purified recombinant-tagged form enzyme | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61852 | - |
x * 61852, calculated from sequence | Mus musculus |
| 68000 | - |
x * 68000, secreted-tagged enzyme form, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | in mammalian cells, polyamine catabolism seems to be mediated by the activity of two enzymes, PAO and SMO | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q99K82 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | in mammalian cells, polyamine catabolism seems to be mediated by the activity of two enzymes, PAO and SMO | Mus musculus | ? | - |
? | |
| additional information | fails to act upon spermidine, N1-acetylpolyamines, putrescine and N1-acetylcadaverine | Mus musculus | ? | - |
? | |
| spermine + O2 + H2O | - |
Mus musculus | spermidine + 3-aminopropanal + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61852, calculated from sequence | Mus musculus |
| ? | x * 68000, secreted-tagged enzyme form, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mSMO | - |
Mus musculus |
| spermine oxidase | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
spermine | pH 8.0, 37°C, purified recombinant-tagged form enzyme | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
in Na-phosphate buffer | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavin/mSMO stoichiometry of 1:1 | Mus musculus | |
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Release 2023.1 (January 2023)
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