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Literature summary for 1.5.3.16 extracted from

  • Bianchi, M.; Amendola, R.; Federico, R.; Polticelli, F.; Mariottin, P.
    Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase mu isoform (2005), FEBS J., 272, 3052-3059.
    View publication on PubMed
Search for more literature for 1.5.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant mSMOmuDELTA (with a deletion of the nuclear domain) in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information purified mutant mSMOmuDELTA (with a deletion of the nuclear domain A) exhibits biochemical properties very similar to that of mSMOalpha and mSMOmu, in particular a pH optimum of 8.5 in 0.1 m Na-phosphate buffer, a Km value of 0.222 mM and a kcat value of 0.0208 per sec. Removal of the nuclear domain amino acid region abolishes proper nuclear targeting of the mSMOmu isoform Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 spermine pH 8.5, mutant mSMOmuDELTA (with a deletion of the nuclear domain A) Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol splice variant mSMOmu is present in both nuclear and cytoplasmic compartments, two short protein domains are responsible for the nuclear localization of splice variant mSMOmu Mus musculus 5829
-
nucleus splice variant mSMOmu is present in both nuclear and cytoplasmic compartments, two short protein domains are responsible for the nuclear localization of splice variant mSMOmu Mus musculus 5634
-

Organism

Organism UniProt Comment Textmining
Mus musculus Q99K82
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinantly produced mSMOmuDELTA (with a deletion of the nuclear domain) Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
spermine + O2 + H2O mutant mSMOmuDELTA (with a deletion of the nuclear domain A) is not active on spermidine, N1-acetylspermidine or N1-acetylspermine Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
mSMOmu splice variant Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0208
-
 spermine pH 8.5, mutant mSMOmuDELTA (with a deletion of the nuclear domain A) Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 mutant mSMOmuDELTA (with a deletion of the nuclear domain) Mus musculus