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Literature summary for 1.5.3.16 extracted from
- Yeats Fms1 is a FAD-utilizing polyamine oxidase (2003), Biochem. Biophys. Res. Commun., 303, 771-776.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FMS1 gene cloned in frame with a C-terminal 6x His tag and expressed as a recombinant protein in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00158 | - |
N1-acetylspermine | pH 9.0, 20°C | Saccharomyces cerevisiae |  |
| 0.01 | - |
spermine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 0.027 | - |
N8-Acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 0.066 | - |
N1-acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein purified by affinity chromatography and extensive dialysis | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N1-acetylspermidine + O2 + H2O | - |
Saccharomyces cerevisiae | putrescine + ? | - |
? | |
| N1-acetylspermine + O2 + H2O | - |
Saccharomyces cerevisiae | N1-acetylspermidine + 3-aminopropanal + H2O2 | - |
? | |
| N8-acetylspermidine + O2 + H2O | - |
Saccharomyces cerevisiae | 1,3-diaminopropane + ? | - |
? | |
| spermine + O2 + H2O | - |
Saccharomyces cerevisiae | spermidine + 3-aminopropanal + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fms1 protein | - |
Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 22.4 | - |
N8-Acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 47 | - |
N1-acetylspermine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 165 | - |
spermine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 684 | - |
N1-acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 841 | - |
N8-Acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 10400 | - |
N1-acetylspermidine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 16500 | - |
spermine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
| 29800 | - |
N1-acetylspermine | pH 9.0, 20°C | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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