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Literature summary for 1.5.3.15 extracted from

  • Shukla, O.P.; Müller, S.; Walter, R.D.
    Polyamine oxidase from Acanthamoeba culbertsoni specific for N8-acetylspermidine. (1992), Mol. Biochem. Parasitol., 51, 91-98.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
spermidine 1 mM, stimulation Acanthamoeba culbertsoni
spermine 1 mM, stimulation Acanthamoeba culbertsoni

General Stability

General Stability Organism
polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days Acanthamoeba culbertsoni

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, slight inhibition Acanthamoeba culbertsoni
8-hydroxyquinoline 1 mM, slight inhibition Acanthamoeba culbertsoni
AgNO3 0.1-1 mM Acanthamoeba culbertsoni
CuSO4 0.1-1 mM Acanthamoeba culbertsoni
diphenyldithiocarbazone 0.001 mM, 58% inhibition Acanthamoeba culbertsoni
EDTA 1 mM, slight inhibition Acanthamoeba culbertsoni
HgCl2 0.1-1 mM Acanthamoeba culbertsoni
hydroxylamine 0.1 mM, 69% inhibition Acanthamoeba culbertsoni
MDL 72527 0.001 mM, 61.5% inhibition; 0.01 mM, 99% inhibition Acanthamoeba culbertsoni
phenylhydrazine 0.1 mM, 95% inhibition Acanthamoeba culbertsoni
ZnSO4 0.1-1 mM Acanthamoeba culbertsoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.105
-
N8-Acetylspermidine 37°C, pH 8 Acanthamoeba culbertsoni
0.338
-
N1-acetylspermine 37°C, pH 8 Acanthamoeba culbertsoni

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 1-10 mM, stimulation Acanthamoeba culbertsoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration Acanthamoeba culbertsoni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N8-acetylspermidine + O2 + H2O Acanthamoeba culbertsoni the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba 1,3-diaminopropanal + H2O2 + ?
-
?

Organism

Organism UniProt Comment Textmining
Acanthamoeba culbertsoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acanthamoeba culbertsoni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.506
-
-
Acanthamoeba culbertsoni

Storage Stability

Storage Stability Organism
-20°C, 7 days, about 20% loss of activity, crude enzyme Acanthamoeba culbertsoni
4°C, 24 h, 90% loss of activity, crude enzyme Acanthamoeba culbertsoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase activity Acanthamoeba culbertsoni ?
-
?
N1,N12-diacetylspermine + O2 + H2O 15% of the activity with N8-acetylspermidine Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
?
N1-acetylspermine + O2 + H2O 40% of the activity with N8-acetylspermidine Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
?
N8-acetylspermidine + O2 + H2O the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba Acanthamoeba culbertsoni 1,3-diaminopropanal + H2O2 + ?
-
?
N8-acetylspermidine + O2 + H2O
-
Acanthamoeba culbertsoni 1,3-diaminopropane + H2O2 + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
Tris-HCl buffer Acanthamoeba culbertsoni