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show all sequences of 1.5.3.15

Polyamine oxidase from Acanthamoeba culbertsoni specific for N8-acetylspermidine.

Shukla, O.P.; Müller, S.; Walter, R.D.; Mol. Biochem. Parasitol. 51, 91-98 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
spermidine
1 mM, stimulation
Acanthamoeba culbertsoni
spermine
1 mM, stimulation
Acanthamoeba culbertsoni
General Stability
General Stability
Organism
polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days
Acanthamoeba culbertsoni
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, slight inhibition
Acanthamoeba culbertsoni
8-hydroxyquinoline
1 mM, slight inhibition
Acanthamoeba culbertsoni
AgNO3
0.1-1 mM
Acanthamoeba culbertsoni
CuSO4
0.1-1 mM
Acanthamoeba culbertsoni
diphenyldithiocarbazone
0.001 mM, 58% inhibition
Acanthamoeba culbertsoni
EDTA
1 mM, slight inhibition
Acanthamoeba culbertsoni
HgCl2
0.1-1 mM
Acanthamoeba culbertsoni
hydroxylamine
0.1 mM, 69% inhibition
Acanthamoeba culbertsoni
MDL 72527
0.001 mM, 61.5% inhibition; 0.01 mM, 99% inhibition
Acanthamoeba culbertsoni
phenylhydrazine
0.1 mM, 95% inhibition
Acanthamoeba culbertsoni
ZnSO4
0.1-1 mM
Acanthamoeba culbertsoni
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.105
-
N8-Acetylspermidine
37°C, pH 8
Acanthamoeba culbertsoni
0.338
-
N1-acetylspermine
37°C, pH 8
Acanthamoeba culbertsoni
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
MgCl2
1-10 mM, stimulation
Acanthamoeba culbertsoni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration
Acanthamoeba culbertsoni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N8-acetylspermidine + O2 + H2O
Acanthamoeba culbertsoni
the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba
1,3-diaminopropanal + H2O2 + ?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acanthamoeba culbertsoni
-
-
-
Purification (Commentary)
Commentary
Organism
-
Acanthamoeba culbertsoni
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.506
-
-
Acanthamoeba culbertsoni
Storage Stability
Storage Stability
Organism
-20°C, 7 days, about 20% loss of activity, crude enzyme
Acanthamoeba culbertsoni
4°C, 24 h, 90% loss of activity, crude enzyme
Acanthamoeba culbertsoni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase activity
694075
Acanthamoeba culbertsoni
?
-
-
-
-
N1,N12-diacetylspermine + O2 + H2O
15% of the activity with N8-acetylspermidine
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
N1-acetylspermine + O2 + H2O
40% of the activity with N8-acetylspermidine
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
N8-acetylspermidine + O2 + H2O
the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba
694075
Acanthamoeba culbertsoni
1,3-diaminopropanal + H2O2 + ?
-
-
-
?
N8-acetylspermidine + O2 + H2O
-
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
Tris-HCl buffer
Acanthamoeba culbertsoni
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
spermidine
1 mM, stimulation
Acanthamoeba culbertsoni
spermine
1 mM, stimulation
Acanthamoeba culbertsoni
General Stability (protein specific)
General Stability
Organism
polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days
Acanthamoeba culbertsoni
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, slight inhibition
Acanthamoeba culbertsoni
8-hydroxyquinoline
1 mM, slight inhibition
Acanthamoeba culbertsoni
AgNO3
0.1-1 mM
Acanthamoeba culbertsoni
CuSO4
0.1-1 mM
Acanthamoeba culbertsoni
diphenyldithiocarbazone
0.001 mM, 58% inhibition
Acanthamoeba culbertsoni
EDTA
1 mM, slight inhibition
Acanthamoeba culbertsoni
HgCl2
0.1-1 mM
Acanthamoeba culbertsoni
hydroxylamine
0.1 mM, 69% inhibition
Acanthamoeba culbertsoni
MDL 72527
0.001 mM, 61.5% inhibition; 0.01 mM, 99% inhibition
Acanthamoeba culbertsoni
phenylhydrazine
0.1 mM, 95% inhibition
Acanthamoeba culbertsoni
ZnSO4
0.1-1 mM
Acanthamoeba culbertsoni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.105
-
N8-Acetylspermidine
37°C, pH 8
Acanthamoeba culbertsoni
0.338
-
N1-acetylspermine
37°C, pH 8
Acanthamoeba culbertsoni
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
MgCl2
1-10 mM, stimulation
Acanthamoeba culbertsoni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration
Acanthamoeba culbertsoni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
N8-acetylspermidine + O2 + H2O
Acanthamoeba culbertsoni
the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba
1,3-diaminopropanal + H2O2 + ?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acanthamoeba culbertsoni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.506
-
-
Acanthamoeba culbertsoni
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 7 days, about 20% loss of activity, crude enzyme
Acanthamoeba culbertsoni
4°C, 24 h, 90% loss of activity, crude enzyme
Acanthamoeba culbertsoni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase activity
694075
Acanthamoeba culbertsoni
?
-
-
-
-
N1,N12-diacetylspermine + O2 + H2O
15% of the activity with N8-acetylspermidine
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
N1-acetylspermine + O2 + H2O
40% of the activity with N8-acetylspermidine
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
N8-acetylspermidine + O2 + H2O
the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba
694075
Acanthamoeba culbertsoni
1,3-diaminopropanal + H2O2 + ?
-
-
-
?
N8-acetylspermidine + O2 + H2O
-
694075
Acanthamoeba culbertsoni
1,3-diaminopropane + H2O2 + ?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
Tris-HCl buffer
Acanthamoeba culbertsoni
Other publictions for EC 1.5.3.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
694075
Shukla
Polyamine oxidase from Acantha ...
Acanthamoeba culbertsoni
Mol. Biochem. Parasitol.
51
91-98
1992
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1
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