Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermidine | 1 mM, stimulation | Acanthamoeba culbertsoni | |
spermine | 1 mM, stimulation | Acanthamoeba culbertsoni |
General Stability | Organism |
---|---|
polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days | Acanthamoeba culbertsoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, slight inhibition | Acanthamoeba culbertsoni | |
8-hydroxyquinoline | 1 mM, slight inhibition | Acanthamoeba culbertsoni | |
AgNO3 | 0.1-1 mM | Acanthamoeba culbertsoni | |
CuSO4 | 0.1-1 mM | Acanthamoeba culbertsoni | |
diphenyldithiocarbazone | 0.001 mM, 58% inhibition | Acanthamoeba culbertsoni | |
EDTA | 1 mM, slight inhibition | Acanthamoeba culbertsoni | |
HgCl2 | 0.1-1 mM | Acanthamoeba culbertsoni | |
hydroxylamine | 0.1 mM, 69% inhibition | Acanthamoeba culbertsoni | |
MDL 72527 | 0.001 mM, 61.5% inhibition; 0.01 mM, 99% inhibition | Acanthamoeba culbertsoni | |
phenylhydrazine | 0.1 mM, 95% inhibition | Acanthamoeba culbertsoni | |
ZnSO4 | 0.1-1 mM | Acanthamoeba culbertsoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
N8-Acetylspermidine | 37°C, pH 8 | Acanthamoeba culbertsoni | |
0.338 | - |
N1-acetylspermine | 37°C, pH 8 | Acanthamoeba culbertsoni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | 1-10 mM, stimulation | Acanthamoeba culbertsoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration | Acanthamoeba culbertsoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N8-acetylspermidine + O2 + H2O | Acanthamoeba culbertsoni | the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba | 1,3-diaminopropanal + H2O2 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthamoeba culbertsoni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acanthamoeba culbertsoni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.506 | - |
- |
Acanthamoeba culbertsoni |
Storage Stability | Organism |
---|---|
-20°C, 7 days, about 20% loss of activity, crude enzyme | Acanthamoeba culbertsoni |
4°C, 24 h, 90% loss of activity, crude enzyme | Acanthamoeba culbertsoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase activity | Acanthamoeba culbertsoni | ? | - |
? | |
N1,N12-diacetylspermine + O2 + H2O | 15% of the activity with N8-acetylspermidine | Acanthamoeba culbertsoni | 1,3-diaminopropane + H2O2 + ? | - |
? | |
N1-acetylspermine + O2 + H2O | 40% of the activity with N8-acetylspermidine | Acanthamoeba culbertsoni | 1,3-diaminopropane + H2O2 + ? | - |
? | |
N8-acetylspermidine + O2 + H2O | the enzyme could be involved in the biosynthesis of diaminopropane which is present inlarge quantities in this amoeba | Acanthamoeba culbertsoni | 1,3-diaminopropanal + H2O2 + ? | - |
? | |
N8-acetylspermidine + O2 + H2O | - |
Acanthamoeba culbertsoni | 1,3-diaminopropane + H2O2 + ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
Tris-HCl buffer | Acanthamoeba culbertsoni |