Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arthrobacter globiformis |
Crystallization (Comment) | Organism |
---|---|
mutant D552A in complex with tetrahydrofolate. Presence of one water molecule instead of the Asp552 side chain | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
D552A | mutation leads to increased formaldehyde release | Arthrobacter globiformis |
additional information | complete removal of 5,10-CH2-THF synthase domain through expression of a truncated version generates a mutant enzyme unable to avoid hydrolysis of the imine species generated following amine oxidation | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + H2O + O2 | the oxidation of dimethylglycine is coupled to the synthesis of 5,10-methylenetetrahydrofolate through an unusual substrate channeling mechanism. Uncoupling of the active sites can be achieved by mutagenesis or deletion of the 5,10-methylenetetrahydrofolate synthase site and this leads to accumulation of intracellular formaldehyde. Channeling occurs by nonbiased diffusion of the labile intermediate through a large solvent cavity connecting both active sites | Arthrobacter globiformis | sarcosine + formaldehyde + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arthrobacter globiformis |