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Literature summary for 1.5.3.10 extracted from
- Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp. Implications for glycine betaine catabolism (2001), Eur. J. Biochem., 268, 3390-3398.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arthrobacter globiformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
N,N-dimethylglycine | - |
Arthrobacter globiformis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
x * 88000, SDS-PAGE | Arthrobacter globiformis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethylglycine + H2O + O2 | Arthrobacter globiformis | involved in tetrahydrofolate-dependent assimilation of methyl groups | sarcosine + formaldehyde + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter globiformis | Q9AGP8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, recombinant enzyme | Arthrobacter globiformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Arthrobacter globiformis | - |
| hepatocyte | - |
Arthrobacter globiformis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N-dimethylglycine + H2O + O2 | involved in tetrahydrofolate-dependent assimilation of methyl groups | Arthrobacter globiformis | sarcosine + formaldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88000, SDS-PAGE | Arthrobacter globiformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.3 | - |
N,N-dimethylglycine | - |
Arthrobacter globiformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Arthrobacter globiformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | 8alpha-N1-histidyl flavin | Arthrobacter globiformis | |
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Release 2023.1 (January 2023)
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