Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.1 extracted from

  • Xin, Y.; Hao, M.; Fan, G.; Zhang, Y.; Zhang, L.
    Soluble expression of Thermomicrobium roseum sarcosine oxidase and characterization of N-demethylation activity (2019), Mol. Catal., 464, 48-56 .
No PubMed abstract available
Search for more literature for 1.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Thermomicrobium roseum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 N-Methyl-L-alanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
61.2
-
 sarcosine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
98
-
 L-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
136.5
-
 N-methyl-L-tryptophan wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
154.3
-
 2-isopropyl-4-(methylaminomethyl)thiazole wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
168.5
-
 N-Methyl-L-leucine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
177.8
-
 N-methyl-L-phenylalanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
244.7
-
 N-methyl-L-aspartate wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
258.4
-
 4-(ethylaminomethyl)pyridine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
324.5
-
 N-methyl-D-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum

Organism

Organism UniProt Comment Textmining
Thermomicrobium roseum B9L163
-
-
Thermomicrobium roseum DSM 5159 B9L163
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2
-
 ?
2-isopropyl-4-(methylaminomethyl)thiazole + O2 + H2O
-
 Thermomicrobium roseum DSM 5159 formaldehyde + 2-isopropyl-4-(aminomethyl)thiazole + H2O2
-
 ?
4-(ethylaminomethyl)pyridine + O2 + H2O
-
 Thermomicrobium roseum acetaldehyde + 4-(aminomethyl) pyridine + H2O2
-
 ?
4-(ethylaminomethyl)pyridine + O2 + H2O
-
 Thermomicrobium roseum DSM 5159 acetaldehyde + 4-(aminomethyl) pyridine + H2O2
-
 ?
L-proline + O2 + H2O
-
 Thermomicrobium roseum ? + H2O2
-
 ?
N-methyl-D-proline + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + D-proline + H2O2
-
 ?
N-methyl-L-alanine + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + L-alanine + H2O2
-
 ?
N-methyl-L-alanine + O2 + H2O
-
 Thermomicrobium roseum DSM 5159 formaldehyde + L-alanine + H2O2
-
 ?
N-methyl-L-aspartate + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + L-aspartate + H2O2
-
 ?
N-methyl-L-leucine + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + L-leucine + H2O2
-
 ?
N-methyl-L-leucine + O2 + H2O
-
 Thermomicrobium roseum DSM 5159 formaldehyde + L-leucine + H2O2
-
 ?
N-methyl-L-phenylalanine + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + L-phenylalanine + H2O2
-
 ?
N-methyl-L-tryptophan + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + L-tryptophan + H2O2
-
 ?
sarcosine + O2 + H2O
-
 Thermomicrobium roseum formaldehyde + glycine + H2O2
-
 ?
sarcosine + O2 + H2O
-
 Thermomicrobium roseum DSM 5159 formaldehyde + glycine + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Thermomicrobium roseum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 melting temperature Thermomicrobium roseum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00004
-
 4-(ethylaminomethyl)pyridine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0003
-
 L-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.004
-
 N-Methyl-L-alanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0045
-
 N-methyl-L-tryptophan wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0048
-
 N-methyl-D-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.009
-
 N-methyl-L-phenylalanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0295
-
 N-methyl-L-aspartate wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0458
-
 2-isopropyl-4-(methylaminomethyl)thiazole wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.053
-
 sarcosine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0537
-
 N-Methyl-L-leucine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000015
-
 N-methyl-D-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.000033
-
 N-methyl-L-tryptophan wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.00004
-
 4-(ethylaminomethyl)pyridine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.000051
-
 N-methyl-L-phenylalanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.00012
-
 N-methyl-L-aspartate wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.00021
-
 N-Methyl-L-leucine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.0003
-
 L-proline wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.00033
-
 2-isopropyl-4-(methylaminomethyl)thiazole wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.00087
-
 sarcosine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum
0.016
-
 N-Methyl-L-alanine wild-type, 37°C, pH not specified in the publication Thermomicrobium roseum