KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics relative to pH, stopped-flow spectroscopy | Corynebacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Corynebacterium sp. | heterotetrameric sarcosine oxidase is a flavoprotein that catalyses the oxidative demethylation of sarcosine to generate glycine, hydrogen peroxide and formaldehyde or 5,10-methylenetetrahydrofolate, depending on the availability of tetrahydrofolate. The amine proton of sarcosine is transferred to the unprotonated Lys residue in the enzyme-substrate complex | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | heterotetrameric sarcosine oxidase is a flavoprotein that catalyses the oxidative demethylation of sarcosine to generate glycine, hydrogen peroxide and formaldehyde or 5,10-methylenetetrahydrofolate, depending on the availability of tetrahydrofolate. The amine proton of sarcosine is transferred to the unprotonated Lys residue in the enzyme-substrate complex | Corynebacterium sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Corynebacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
SO-U96 | - |
Corynebacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Corynebacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 10.5 | activity range | Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, rate of FAD reduction, overview. Reduction of the bound FAD cofactor occurs, the electron transfers from the reduced FAD to the bound FMN cofactor, then the oxidized FAD is reduced again following EoxS complex formation | Corynebacterium sp. | |
FMN | flavoprotein, overview. Reduction of the bound FAD cofactor occurs, the electron transfers from the reduced FAD to the bound FMN cofactor, then the oxidized FAD is reduced again following EoxS complex formation | Corynebacterium sp. |