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Literature summary for 1.5.3.1 extracted from

  • Matsuda, Y.; Hoshika, H.; Inouye, Y.; Ikuta, S.; Matsuura, K.; Nakamura, S.
    Purification and characterization of sarcosine oxidase of Bacillus origin (1987), Chem. Pharm. Bull., 35, 711-717.
    View publication on PubMed
Search for more literature for 1.5.3.1

Inhibitors

Inhibitors Comment Organism Structure
EDTA not Bacillus sp. (in: Bacteria)
Fe3+
-
 Bacillus sp. (in: Bacteria)
N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)
p-chloromercuribenzoate not inhibitory Bacillus sp. (in: Bacteria)
Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.8
-
 N-Methyl-DL-alanine
-
 Bacillus sp. (in: Bacteria)
12.2
-
 sarcosine
-
 Bacillus sp. (in: Bacteria)
106
-
 N-Methyl-L-leucine
-
 Bacillus sp. (in: Bacteria)
173
-
 N-Methyl-DL-valine
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 B-0618
-
Bacillus sp. (in: Bacteria) B-0618
-
 B-0618
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: beta-alanine, N-methylalanine, 1,3-dimethylurea, 1-methylguanidine, methoxyacetate, creatine, creatinine Bacillus sp. (in: Bacteria) ?
-
 ?
additional information not: beta-alanine, N-methylalanine, 1,3-dimethylurea, 1-methylguanidine, methoxyacetate, creatine, creatinine Bacillus sp. (in: Bacteria) B-0618 ?
-
 ?
N-methyl-DL-alanine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) formaldehyde + DL-alanine + H2O2
-
 ?
N-methyl-DL-alanine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) B-0618 formaldehyde + DL-alanine + H2O2
-
 ?
N-methyl-DL-valine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) formaldehyde + DL-valine + H2O2
-
 ?
N-methyl-DL-valine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) B-0618 formaldehyde + DL-valine + H2O2
-
 ?
N-methyl-L-leucine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) formaldehyde + L-leucine + H2O2
-
 ?
N-methyl-L-leucine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) B-0618 formaldehyde + L-leucine + H2O2
-
 ?
sarcosine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?
sarcosine + O2 + H2O
-
 Bacillus sp. (in: Bacteria) B-0618 glycine + formaldehyde + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 7-9, 10 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus sp. (in: Bacteria)
8.5 9
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 labile below Bacillus sp. (in: Bacteria)
7 10
-
 Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
flavin
-
 Bacillus sp. (in: Bacteria)