Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.B7 extracted from

  • McFarlane, J.S.; Lamb, A.L.
    Biosynthesis of an opine metallophore by Pseudomonas aeruginosa (2017), Biochemistry, 56, 5967-5971 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene cntM, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten steady-state kinetics Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa ATCC 15692 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa 1C i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa PRS 101 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa DSM 22644 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa CIP 104116 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa LMG 12228 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ Pseudomonas aeruginosa JCM 14847 i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine pseudopaline + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HUX5
-
-
Pseudomonas aeruginosa 1C Q9HUX5
-
-
Pseudomonas aeruginosa ATCC 15692 Q9HUX5
-
-
Pseudomonas aeruginosa CIP 104116 Q9HUX5
-
-
Pseudomonas aeruginosa DSM 22644 Q9HUX5
-
-
Pseudomonas aeruginosa JCM 14847 Q9HUX5
-
-
Pseudomonas aeruginosa LMG 12228 Q9HUX5
-
-
Pseudomonas aeruginosa PRS 101 Q9HUX5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme PaODH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration, to over 95% purity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa ATCC 15692 pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa 1C pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa PRS 101 pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa DSM 22644 pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa CIP 104116 pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa LMG 12228 pseudopaline + NADP+ + H2O
-
?
(2S)-2-amino-4-([(1S)-1-carboxy-2-(1H-imidazol-4-yl)ethyl]amino)butanoate + oxaloacetate + NADPH + H+ i.e. N-[(3S)-3-amino-3-carboxypropyl]-L-histidine Pseudomonas aeruginosa JCM 14847 pseudopaline + NADP+ + H2O
-
?
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa ATCC 15692 ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa 1C ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa PRS 101 ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa DSM 22644 ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa CIP 104116 ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa LMG 12228 ?
-
-
additional information Pseudomonas aeruginosa ODH shows no catalytic activity in the presence of pyruvate or oxaloacetate (within error of zero), but full activity with 2-oxoglutarate. Coupled assay with enzyme nicotianamine synthase from Pseudomonas aeruginosa (PaNAS) and L-histidine. Substrate specificities, metabolite analysis by NMR, overview Pseudomonas aeruginosa JCM 14847 ?
-
-

Synonyms

Synonyms Comment Organism
cntM
-
Pseudomonas aeruginosa
ODH
-
Pseudomonas aeruginosa
PA4835
-
Pseudomonas aeruginosa
PaODH
-
Pseudomonas aeruginosa
pseudopaline synthase UniProt Pseudomonas aeruginosa

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
22
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
additional information lower affinity of PaODH for NADH, preferably PaODH binds NADPH Pseudomonas aeruginosa
NADPH
-
Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism the NADPH is oxidized to NADP+ by the PaODH in the presence of Pseudomonas aeruginosa nicotianamine synthase (PaNAS), S-adenosyl-L-methionine (SAM) and the correct amino acid and 2-oxo acid substrates. Pyruvate, oxaloacetate, and 2-oxoglutarate are screened with SAM, but no oxidation is observed, suggesting that SAM alone is not sufficient as a substrate for PaNAS. Screening of 42 L- and D-amino acid substrates in combination with pyruvate, oxaloacetate, or 2-oxoglutarate reveals that several amino acids, L-threonine, L-asparagine, and L-hydroxyproline (L-Hyp), show limited turnover, while the combination of L-histidine and 2-oxoglutarate results in significant oxidation of NADPH by PaODH. PaNAS is specific for L-histidine Pseudomonas aeruginosa
physiological function opine dehydrogenases (ODHs) typically form a secondary amine by condensation of an amino acid with an alpha-keto acid. Pseudomonas aeruginosa encodes the enzymes nicotianamine synthase (NAS) and opine dehydrogenase (ODH), biosynthesizing the nicotianamine-like opine metallophore pseudopaline Pseudomonas aeruginosa